Transcriptomic Analysis of Triticum aestivum under Salt Stress Reveals Change of Gene Expression

Donghyun Jeon; Yoonho Lim; Yuna Kang; Chulsoo Park; Donghoon Lee; Junchan Park; Uchan Choi; Kyeonghoon Kim; Changsoo Kim

doi:10.7740/kjcs.2022.67.1.041

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2022 Vol.67, Issue 1 Preview Page Next Page

Original Research Article

Transcriptomic Analysis of Triticum aestivum under Salt Stress Reveals Change of Gene Expression RNA sequencing을 이용한 염 스트레스 처리 밀(Triticum aestivum)의 유전자 발현 차이 확인 및 후보 유전자 선발

1 March 2022. pp. 41-52

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Abstract

As a cultivar of Korean wheat, ‘Keumgang’ wheat variety has a fast growth period and can be grown stably. Hexaploid wheat (Triticum aestivum) has moderately high salt tolerance compared to tetraploid wheat (Triticum turgidum L.). However, the molecular mechanisms related to salt tolerance of hexaploid wheat have not been elucidated yet. In this study, the candidate genes related to salt tolerance were identified by investigating the genes that are differently expressed in Keumgang variety and examining salt tolerant mutation ‘2020-s1340.’. A total of 85,771,537 reads were obtained after quality filtering using NextSeq 500 Illumina sequencing technology. A total of 23,634,438 reads were aligned with the NCBI Campala Lr22a pseudomolecule v5 reference genome (Triticum aestivum). A total of 282 differentially expressed genes (DEGs) were identified in the two Triticum aestivum materials. These DEGs have functions, including salt tolerance related traits such as ‘wall-associated receptor kinase-like 8’, ‘cytochrome P450’, ‘6-phosphofructokinase 2’. In addition, the identified DEGs were classified into three categories, including biological process, molecular function, cellular component using gene ontology analysis. These DEGs were enriched significantly for terms such as the ‘copper ion transport’, ‘oxidation-reduction process’, ‘alternative oxidase activity’. These results, which were obtained using RNA-seq analysis, will improve our understanding of salt tolerance of wheat. Moreover, this study will be a useful resource for breeding wheat varieties with improved salt tolerance using molecular breeding technology.

Keywords

candidate gene

differentially expressed gene

gene ontology

salt tolerance

wheat

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Information

Publisher :The Korean Society of Crop Science
Publisher(Ko) :한국작물학회
Journal Title :The Korean Journal of Crop Science
Journal Title(Ko) :한국작물학회지
Volume : 67
No :1
Pages :41-52
Received Date : 2022-01-20
Revised Date : 2022-02-08
Accepted Date : 2022-02-08
DOI :https://doi.org/10.7740/kjcs.2022.67.1.041

The Korean Journal of Crop Science ISSN:0252-9777(Print) 2287-8432(Online) 한국작물학회지

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