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Original Research Article

Effects of Elicitors on Seedling Growth, Total Polyphenol and Chlorophyll Content and Antioxidant Activity of Barley (Hordeum vulgare L.)
Timnoy Salitxay1
,
Phonesavanh Phouthaxay1
,
Yeoun-Gyu Pang2
,
Yu-Chi Yeong2
,
Prakash Babu Adhikari3
,
Cheol-Ho Park1*
1Department of Bio - Health Technology, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea
2Plant Square Co. Chuncheon 200-000, Korea
3Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 200-701, Korea
*Corresponding Author
31 March 2016. pp. 33-40
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Abstract

This study is focused on the evaluation of growth parameters, total polyphenol content (TPC), chlorophyll content as well as the DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity of young barley seedling (YBS) affected by elicitation. Salicylic acid (SA), methyl jasmonate (MJ), amino acid liquid fertilizer (ALF) and microbial metabolism activator (MMA) were used. Elicitation was conducted for two times and various concentrations were used in this study. The result revealed that, MJ 1 ml/L treated-YBS gave the longest seedling length of 1.33 cm, followed by the ones treated with SA 1.38 mg/L and ALF 2 ml/L, respectively. ALF 3 ml/L treatment gave the highest fresh weight of 10 seedlings, followed by MJ 5 ml/L and SA 13.8 mg/L treatment with 1.56 g, 1.55 g and 1.53 g respectively. SA 138.12 mg/L elicitor treated-YBS gave the highest Chl a, Chl b content of 8.57 μg/mg and 3.83 μg/mg, respectively while the highest carotenoid content was found in MJ ml/L treatment with 1.62 μg/mg. Among elicitor treated-YBS, SA showed better TPC. The highest TPC was found in SA 1.38 mg/L treatment with 18.82 mg/g TAE. Likewise, SA 1.38 mg/L showed the highest DPPH free radical scavenging activity among all the treatments. However, the lowest TPC was found in ALF 1ml/L treated-YBS with 9.46 mg/g TAE, which was even lower than the control (14.31 mg/g TAE).

Keywords
Bayley (Hordeum vulgare L)
Chlorophyll
DPPH (1,1-diphenyl-2-picryhydrazyl) free radical scavenging activity
Elicitation
Growth parameters
Total polyphenol
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 61
  • No :1
  • Pages :33-40
  • Received Date : 2015-12-22
  • Revised Date : 2016-02-11
  • Accepted Date : 2016-02-17
  • DOI :https://doi.org/10.7740/kjcs.2016.61.1.033
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