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2019 Vol.64, Issue 4 Preview Page

Original Research Article

Identification of the Protein Function and Comparison of the Protein Expression Patterns of Wheat Addition Lines with Wild Rye Chromosomes

야생 호밀 염색체 첨가 밀 계통의 단백질 발현 양상 비교 분석

Dae Han Lee1
,
Kun Cho2
,
Sun Hee Woo3
,
Seong-Woo Cho4*
이 대한1
,
조 건2
,
우 선희3
,
조 성우4*
1Staff, DAEYU Co., LTD,
2Senior Researcher, Center for Research Equipment, Korean Basic Science Institute
3Professor, Department of Crop Science, Chungbuk National University
4Assistant Professor, Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science and Technology
1㈜대유 마케팅부 사원
2한국기초과학지원연구원 연구장비운영부 선임연구원
3충북대학교 식물자원학과 교수
4경남과학기술대학교 농학‧한약자원학부 조교수
* Corresponding Author
31 December 2019. pp. 373-383
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Abstract

The objectives of this study were to compare the protein expression patterns and degrees and identify the protein function of disomic addition lines (DAs) in Leymus racemosus, in order to improve the quality of wheat. Upon SDS-PAGE, L. racemosus showed two major protein bands whereas Chinese Spring (CS) had four major protein bands of high molecular weight. The DA(s) generally showed a similar protein expression pattern to that of CS, because 42 chromosomes were from CS and two chromosomes were from L. racemosus. However, only the L.r[J] line showed two protein bands of between 15 and 20 kDa, like L. racemosus. Image analysis based on 2-DE revealed that L.r[F] had the most upregulated protein spots, whereas L.r[N] had the least upregulated protein spots. For L.r[I], the frequency of the downregulated protein spots was higher than that of the upregulated ones. Using MALDI-TOF MS, the protein function was identified for each protein spot on the 2-DE polyacrylamide gel. The protein spots were classified into 11 groups according to protein function. Among the 11 groups, most protein spots of the DA(s) were identified as proteins related to metabolism. Additionally, unique protein spots of the DA(s) were related to abiotic stressors such as cold and heat. Those proteins are useful for improving wheat quality with resistance against abiotic stressors.

Keywords
disomic addition lines
Leymus
protein
proteomics
wheat
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 64
  • No :4
  • Pages :373-383
  • Received Date : 2019-10-14
  • Revised Date : 2019-10-23
  • Accepted Date : 2019-10-25
  • DOI :https://doi.org/10.7740/kjcs.2019.64.4.373
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