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2025 Vol.70, Issue 2 Preview Page

Original Research Article

Physiological Activities of Extracts from Common Buckwheat (Fagopyrum esculentum Moench.) Leaves Using Different Extraction Methods

에탄올추출물과 열수추출물에 따른 일반메밀 잎의 생리활성

Sun-Hee Woo1
,
Sung-Hyun Yun2
,
Mittra Probir Kumar2
,
Ju-Young Choi2
,
Young-Hwan Ju2
,
Hyun-Jin Jung3
,
Ju-Ho Kim4
,
Hag Hyun Kim5
,
Tae-Hyung Kim5
,
Min-Jeong Lee6
,
Tae-Ho Kim7
,
Soo-Jeong Kwon2*
,
Tae-Young Hwang8*
우 선희1
,
윤 성현2
,
Kumar Mittra Probir2
,
최 주영2
,
주 영환2
,
정 현진3
,
김 주호4
,
김 학현5
,
김 태형5
,
이 민정6
,
김 태호7
,
권 수정2*
,
황 태영8*
1Professor, Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea
2Researcher, Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea
3Postdoctoral Researcher, National Agricultural Satellite Center, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 54875, Republic of Korea
4Graduate Student, Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea
5Professor, Department of Culinary Arts, Woosong College, Daejeon 34606, Republic of Korea
6Senior Researcher, R&D Headquaters, Korea Ginseng Corporation, 30, Gajeong-ro, Yuseong-gu, Deajeon 34128, Republic of Korea
7Senior Researcher, Breeding Research Center, KWONNONG SEED Co.,Ltd. Cheongju 28174, Republic of Korea
8Associate Professor, Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea
1충북대학교 식물자원학과 교수
2충북대학교 식물자원학과 연구원
3농촌진흥청 국립농업과학원, 농업위성센터 전문연구원
4충북대학교 식물자원학과 대학원생
5우송정보대학 외식조리학부 교수
6한국인삼공사 R&D본부 선임연구원
7농업회사법인 권농종묘(주), 육종연구소 주임연구원
8충북대학교 식물자원학과 부교수
*Corresponding Author
1 June 2025. pp. 104-112
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Abstract

This study investigated the antioxidant activity of common buckwheat (Fagopyrum esculentum Moench.) leaves using various ethanol-based extraction methods. The leaf extracts were obtained using hot water and 50%, 70%, and 100% (v/v) ethanol, and were evaluated for total polyphenol and flavonoid contents. Antioxidant and biological activities were assessed using DPPH and ABTS radical scavenging assays, nitrite scavenging activity, cell viability, nitric oxide (NO) production, LPS-induced IL-1β, IL-6, and TNF-α production in RAW 264.7 cells, and tyrosinase inhibition activity. The total polyphenol and flavonoid contents were highest in the 100% ethanol extract, with values of 297.35 mg/g and 209.56 mg/g, respectively. The DPPH radical scavenging activity increased with extract concentration, reaching 97.23% at 10 mg/mL in the 100% ethanol extract. Similarly, ABTS radical scavenging activity was highest in the 100% ethanol extract, reaching 97.17% at 20 mg/mL, with a concentration- dependent trend. The nitrite scavenging activity was also highest in the 100% ethanol extract, showing 65.9% activity at pH 1.2, and decreased as the pH increased. NO production in macrophages was more suppressed in ethanol extracts than in the hot water extract, with higher concentrations inducing greater NO inhibition. Cytokine analysis showed that buckwheat leaf extracts reduced the secretion of IL-1β, IL-6, and TNF-α, indicating significant anti-inflammatory activity, particularly in the 100% ethanol extract, which showed slightly higher activity than the 70% and 50% ethanol and hot water extracts. The tyrosinase- inhibiting effect was measured for each extraction solvent in common buckwheat. A lower concentration in the extraction solvent resulted in higher tyrosinase activity, indicating solvent-specific characteristics.

Keywords
anti-inflammatory activity
antioxidant activity
common buckwheat
ethanol extraction
tyrosinase inhibition
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 70
  • No :2
  • Pages :104-112
  • Received Date : 2025-04-17
  • Revised Date : 2025-05-09
  • Accepted Date : 2025-05-09
  • DOI :https://doi.org/10.7740/kjcs.2025.70.2.104
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