Peptide Profiling and Selection of Specific-Expressed Peptides in Hypoglycemic Sorghum Seed using SELDI-TOF MS

Sei Joon Park; Su Min Hwang; Jun Young Park; Jee-Yeon Ko; Tae Wan Kim

doi:10.7740/kjcs.2014.59.3.252

All Issue

2014 Vol.59, Issue 3 Preview Page Next Page

Original Research Article

Peptide Profiling and Selection of Specific-Expressed Peptides in Hypoglycemic Sorghum Seed using SELDI-TOF MS SELDI-TOF MS를 활용한 혈당강하 수수 종자의 펩타이드 프로파일링 및 특이 발현 펩타이드 선발

30 September 2014. pp. 252-262

PDF XML

Abstract

Sorghum seed is traditionally used as secondary food sources in addition to rice in Korea. While the hypoglycemia regulating phytochemicals have been found in sorghum seed, peptides related with hypoglycemia never been studied before. To obtain the peptide characteristics and the specifically high-expressed peptides in hypoglycemic sorghum seed, peptide profiles of seven hypoglycemic and five nonhypoglycemic sorghum lines bred in RDA were determined using surface-enhanced laser desorption/ionization time-offlight mass spectrometry (SELDI-TOF MS). The twelve sorghum lines exhibited 104 peptides on CM10 protein chip array (weak cation exchange) and 95 peptides on Q10 (weak cation exchange) in the molecular mass range from 2,000 to 20,000 Da. Heat map via supervised hierarchical clustering of the significantly different peptides (p < 0.01) in peak intensity among the 12 lines effectively revealed the specifically upregulated peptides in each line and distinguished between 7 hypoglycemic and 5 non-hypoglycemic lines. Through the comparison with hypoglycemic and non-hypoglycemic lines, 10 peptides including 2231.6, 2845.4, 2907.9, 3063.5, 3132.6, 3520.8, 4078.8, 5066.2, 5296.5, 5375.5 Da were specifically high-expressed in hypoglycemic lines at p < 0.00001. This study characterized seed peptides of 12 sorghums and found ten peptides highly expressed for hypoglycemic sorghum lines, which could be used as peptide biomarkers for identification of hypoglycemic sorghum.

Keywords

sorghum

seed

peptide

hypoglycemic

SELDI-TOF MS

References

인용문헌(REFERENCES)

PS Belton and JRN Taylor, Trends in Food Science & Technology, Sorghum and millets: protein sources for Africa, 15; 94-98 (2004)

MM Bradford, Anal Biochem, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, 72; 248-54 (1976)

I Camargo Filho, DAG Cortez, T Ueda-Nakamura, CV Nakamura and BP Dias Filho, Phytomedicine, Antiviral activity and mode of action of a peptide isolated from Sorghum bicolor, 15; 202-8 (2008)

L Dykes and LW Rooney, J Cereal Sci, Sorghum and millet phenols and antioxidants, 44; 236-251 (2006)

K Emami, NJ Morris, SJ Cockell, G Golebiowska, QY Shu and AM Gatehouse, J Agric Food Chem, Changes in protein expression profiles between a low phytic acid rice (Oryza sativa L. Ssp. japonica) line and its parental line: a proteomic and bioinformatic approach, 58; 6912-22 (2010)

N Farrokhi, JP Whitelegge and JA Brusslan, Plant Biotechnol J, Plant peptides and peptidomics, 6; 105-34 (2008)

B R Hamaker, AA Mohamed, JE Habben, CP Huang and BA Larkins, Cereal Chem, Efficient procedure for extracting maize and sorghum kernel proteins reveals higher prolamin contents than the conventional method, 72; 583-588 (1995)

R Hartmann and H Meisel, Curr Opin Biotechnol, Food-derived peptides with biological activity: from research to food applications, 18; 163-9 (2007)

HJ Issaq, TD Veenstra, TP Conrads and D Felschow, Biochem Biophys Res Commun, The SELDI-TOF MS approach to proteomics: protein profiling and biomarker identification, 292; 587-92 (2002)

HS Jeon, IM Chung, KH Ma, EH Kim, SJ Young and JK Ahn, Korean J Crop Sci, Analysis of Phenolic Compounds in Sorghum, Foxtail Millet and Common Millet, 56; 361-74 (2011)

V Kamath, S Niketh, A Chandrashekar and PS Rajini, Food Chem, Chymotryptic hydrolysates of α-kafirin, the storage protein of sorghum (Sorghum bicolor) exhibited angiotensin converting enzyme inhibitory activity, 100; 306-311 (2007)

HY Ki, ES Seong, BK Ghimire, IM Chung, SS Kwon, EJ Goh, K Heo, MJ Kim, JD Lim, D Lee and CY Yu, Food Chem, Antioxidant and antimicrobial activities of crude sorghum extract, 115; 12341-239 (2009)

WS Kim, S Jang and HB Krishnan, Crop Sci, Accumulation of Leginsulin, a Hormone-Like Bioactive Peptide, is Drastically Higher in Asian than in North American Soybean Accessions, 52; 262-71 (2012)

PC Mincoff, DA Garcia Cortez, T Ueda-Nakamura, CV Nakamura and BP Dias Filho, Research in Microbiology, Isolation and characterization of a 30kD antifungal protein from seeds of Sorghum bicolor, 157; 326-332 (2006)

M Ndao, A Rainczuk, MC Rioux, TW Spithill and BJ Ward, Trends in Parasitology, Is SELDI-TOF a valid tool for diagnostic biomarkers, 26; 561-67 (2010)

TB Ng, Peptides, Antifungal proteins and peptides of leguminous and non-leguminous origins, 25; 1215-22 (2004)

J H Park, SH Lee, IM Chung and Y Park, Nutr Res Pract, Sorghum extract exerts an anti-diabetic effect by improving insulin sensitivity via PPAR-gamma in mice fed a high-fat diet, 6; 322-7

SH Park and SR Bean, J Agric Food Chem, Investigation and optimization of the factors influencing sorghum protein extraction, 51; 7050-4 (2003)

SJ Park, JY Park, YH Lee, SM Hwang, AR Kim, JY Ko and TW Kim, Korean J Crop Sci, Optimization of SELDI-TOF MS for Peptide Profiling of Sorghum Seed, 58(1); 50-56 (2013)

SJ Park, YH Lee, YJ Nam, BK Baik and TW Kim, Crop Sci, Peptide-Based Biomarker Discovery for Identification of Rice Cultivars using Surface Enhanced Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry, 53; 987-95 (2013)

EF Petricoin and LA Liotta, Curr Opin Biotechnol, SELDI-TOF-based serum proteomic pattern diagnostics for early detection of cancer, 5; 24-30 (2004)

G Povero, M Papale, G Loreto, A Alpi, P Perata and E Loreti, Am J Enol Vitic, Identification of Grapevine Cultivar Biomarkers Using Surface-Enhanced Laser Desorption and Ionization (SELDI-TOF-MS), 61; 492-97 (2010)

Rural Development Administration, Development of Health Supplement Functional Material for Blood Sugar Decrease in Sorghum (2013)

BH Sarmadi and A Ismail, Peptides, Antioxidative peptides from food proteins: a review, 31; 1949-56 (2010)

MS Seo, JY Ko, SB Song, JS Lee, JR Kang, DY Kwak, BG Oh, YN Yoon, MH Nam, HS Jeong and KS Woo, J. Korean Soc. Food Sci. Nutr, Antioxidant Compounds and Activities of Foxtail Millet/ Proso Millet and Sorghum with Different Pulverizing Methods, 40; 790-7 (2011)

C Silva-Sanchez, AP de la Rosa, M F Leon-Galvan, BO de Lumen, A de Leon-Rodriguez and EG de Mejia, J Agric Food Chem, Bioactive peptides in amaranth (Amaranthus hypochondriacus) seed, 56; 1233-40 (2008)

Information

Publisher :The Korean Society of Crop Science
Publisher(Ko) :한국작물학회
Journal Title :The Korean Journal of Crop Science
Journal Title(Ko) :한국작물학회지
Volume : 59
No :3
Pages :252-262
Received Date : 2014-01-16
Revised Date : 2014-02-17
Accepted Date : 2014-02-26
DOI :https://doi.org/10.7740/kjcs.2014.59.3.252

The Korean Journal of Crop Science ISSN:0252-9777(Print) 2287-8432(Online) 한국작물학회지

All Issue

인용문헌(REFERENCES)