Discrimination and Hordein Polypeptide Patterns of Malting Barley Varieties Using UPLC

Young-Mi Yoon; Yang-Kil Kim; Chon-Sik Kang; Jin-Cheon Park; Tae-Il Park

doi:10.7740/kjcs.2021.66.4.326

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2021 Vol.66, Issue 4 Preview Page Next Page

Original Research Article

Discrimination and Hordein Polypeptide Patterns of Malting Barley Varieties Using UPLC UPLC 분석을 이용한 맥주보리 품종의 호데인 단백질 분석 및 품종 판별

1 December 2021. pp. 326-338

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Abstract

Ultra-performance liquid chromatography (UPLC) was used to assess the hordein protein fraction of malting barley. C-hordeins (barley prolamins) were extracted with 70% ethanol (EtOH) and 55% isopropyl alcohol (IPA, 2-propanol), and B-hordeins were extracted with the same alcohols in 1.0% dithiothreitol (DTT). High molecular weight (HMW) prolamins (D-hordeins) were extracted with 50% IPA with 1M Tris-HCl (pH 8.0). The same protein patterns were observed in both the experimental extraction solutions (EtOH and IPA). However, the patterns of hordein, extracted with EtOH and IPA containing 1.0% DTT, differed slightly. C- and B-hordeins extracted from those solutions were analyzed. Twenty-six malting barley varieties developed in Korea were analyzed using UPLC. The varieties were divided into seven groups according to hordein patterns of retention time 16 min to 18 min, and 20 varieties showed unique patterns.

Keywords

hordein

malting barley

UPLC

variety

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Information

Publisher :The Korean Society of Crop Science
Publisher(Ko) :한국작물학회
Journal Title :The Korean Journal of Crop Science
Journal Title(Ko) :한국작물학회지
Volume : 66
No :4
Pages :326-338
Received Date : 2021-10-08
Revised Date : 2021-11-06
Accepted Date : 2021-11-15
DOI :https://doi.org/10.7740/kjcs.2021.66.4.326

The Korean Journal of Crop Science ISSN:0252-9777(Print) 2287-8432(Online) 한국작물학회지

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