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2015 Vol.60, Issue 1 Preview Page

Original Research Article

Proteome Profiling Unfurl Differential Expressed Proteins from Various Explants in Platycodon grandiflorum
Hye-Rim Kim1*
,
Soo-Jeong Kwon2*
,
Swapan Kumar Roy1*
,
Seong-Woo Cho3
,
Hag-Hyun Kim2
,
Kab-Yeon Cho2
,
Hee-Ock Boo4
,
Sun-Hee Woo1**
1Dept. of Crop Science, Chungbuk National University, Cheong-ju 361-763, Korea
2Dept. of Food Nutrition and Cookery, Woosong College, Daejeon, 300-715, Korea
3Rice Research Division, National Institute of Crop Science RDA, Suwon 441-857, Korea
4WellPhyto Co. Ltd., BI Center, GIST, Gwangju 500-712, Korea
*First Author
**Corresponding Author
31 March 2015. pp. 97-106
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Abstract

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (≥ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose- 1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

Keywords
Platycodon grandiflorum
proteome profiling
functional categorization
pharmacologic value
References

REFERENCES

1
KS Ahn, EJ Noh, HL Zhao, SH Jung, SS Kang and YS Kim, Life Sciences, Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-κB activation in RAW 264.7 cells, 76; 2315-2328 (2005)
2
S Bandhakavi, YM Kim, SH Ro, H Xie, G Onsongo, CB Jun, DH Kim and TJ Griffin, Molecular & Cellular Proteomics, Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response, 9; 403-414 (2010)
3
DM Cavalier, O Lerouxel, L Neumetzler, K Yamauchi, A Reinecke, G Freshour, OA Zabotina, MG Hahn, I Burgert and M Pauly, The Plant Cell Online, Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component, 20; 1519-1537 (2008)
4
JH Choi, YP Hwang, HS Lee and HG Jeong, Food and chemical toxicology, Inhibitory effect of Platycodi Radix on ovalbumin-induced airway inflammation in a murine model of asthma, 47; 1272-1279 (2009)
5
YH Choi, DS Yoo, MR Cha, CW Choi, YS Kim, SU Choi, KR Lee and SY Ryu, Journal of natural products, Antiproliferative effects of saponins from the roots of Platycodon grandiflorum on cultured human tumor cells, 73; 1863-1867 (2010)
6
G Decker, G Wanner, MH Zenk and F Lottspeich, Electrophoresis, Characterization of proteins in latex of the opium poppy (Papaver somniferum) using two dimensional gel electrophoresis and microsequencing, 21; 3500-3516 (2000)
7
RJ Deshaies and CA Joazeiro, Annual review of biochemistry, RING domain E3 ubiquitin ligases, 78; 399-434 (2009)
8
A Faik, NJ Price, NV Raikhel and K Keegstra, Proceedings of the National Academy of Sciences, An Arabidopsis gene encoding an ?-xylosyltransferase involved in xyloglucan biosynthesis, 99; 7797-7802 (2002)
9
C Finnie, S Melchior, P Roepstorff and B Svensson, Plant Physiology, Proteome analysis of grain filling and seed maturation in barley, 129; 1308-1319 (2002)
10
CH Foyer, AJ Bloom, G Queval and G Noctor, Annual review of plant biology, Photorespiratory metabolism: genes, mutants, energetics, and redox signaling, 60; 455-484 (2009)
11
CR Goward and DJ Nicholls, Protein Science, Malate dehydrogenase: a model for structure, evolution, and catalysis, 3; 1883-1888 (1994)
12
LK Han, BJ Xu, Y Kimura, YN Zheng and H Okuda, The Journal of nutrition, Platycodi radix affects lipid metabolism in mice with high fat diet-induced obesity, 130; 2760-2764 (2000)
13
YC Hung, PW Wang and TL Pan, Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, Functional proteomics reveal the effect of Salvia miltiorrhiza aqueous extract against vascular atherosclerotic lesions, 1804; 1310-1321 (2010)
14
DI Jacobs, M Gaspari, J van der Greef, R van der Heijden and R Verpoorte, Planta, Proteome analysis of the medicinal plant Catharanthus roseus, 221; 690-704 (2005)
15
AHM Kamal, ID Jang, DE Kim, T Suzuki, KY Chung, JS Choi, MS Lee, CH Park, SU Park, SH Lee, HS Jeong and SH Woo, J. Plant Biol, Proteomics Analysis of Embryo and Endosperm from Mature Common Buckwheat Seeds, 54; 81-91 (2011)
16
N Kenmochi, T Kawaguchi, S Rozen, E Davis, N Goodman, TJ Hudson, T Tanaka and DC Page, Genome research, A map of 75 human ribosomal protein genes, 8; 509-523 (1998)
17
J Kennedy, Clinical therapeutics, Herb and supplement use in the US adult population, 27; 1847-1858 (2005)
18
T Khanal, JH Choi, YP Hwang, YC Chung and HG Jeong, Food and chemical toxicology, Protective effects of saponins from the root of Platycodon grandiflorum against fatty liver in chronic ethanol feeding via the activation of AMP-dependent protein kinase, 47; 2749-2754 (2009)
19
JY Kim, YP Hwang, DH Kim, EH Han, YC Chung, SH Roh and HG Jeong, Bioscience, Biotechnology and biochemistry, Inhibitory effect of the saponins derived from roots of Platycodon grandiflorum on carrageenan induced inflammation, 70; 858-864 (2006)
20
KS Kim, O Ezaki, S Ikemoto and H Itakura, Journal of nutritional science and vitaminology, Effects of Platycodon grandiflorum feeding on serum and liver lipid concentrations in rats with diet-induced hyperlipidemia, 41; 485-491 (1995)
21
S Kosugi and Y Ohashi, The Plant Journal, DNA binding and dimerization specificity and potential targets for the TCP protein family, 30; 337-348 (2002)
22
JY Lee, JW Yoon, CT Kim and ST Lim, Phytochemistry, Antioxidant activity of phenylpropanoid esters isolated and identified from Platycodon grandiflorum A. DC, 65; 3033-3039 (2004)
23
TH Lin and CL Hsieh, Review Pharmacological effects of Salvia miltiorrhiza (Danshen) on cerebral infarction (2010)
24
JY Lu, YY Lin, J Qian, SC Tao, J Zhu, C Pickart and H Zhu, Molecular & Cellular Proteomics, Functional dissection of a HECT ubiquitin E3 ligase, 7; 35-45 (2008)
25
R Mittler and BA Zilinskas, The Plant Journal, Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought, 5; 397-405 (1994)
26
S Mukherjee and M Choudhuri, Physiologia plantarum, Implications of water stressinduced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings, 58; 166-170 (1983)
27
MH Nam, SI Kim, JR Liu, DC Yang, YP Lim, KH Kwon, JS Yoo and YM Park, Journal of Chromatography B, Proteomic analysis of Korean ginseng (Panax ginseng CA Meyer), 815; 147-155 (2005)
28
R Nawrot, A Kalinowski and A Gozdzicka-Jozefiak, Phytochemistry, Proteomic analysis of Chelidonium majus milky sap using twodimensional gel electrophoresis and tandem mass spectrometry, 68; 1612-1622 (2007)
29
CJ Ning, N Ding, GL Wu, HJ Meng, YN Wang and QH Wang, Plant Physiology and Biochemistry, Proteomics research on the effects of applying selenium to apple leaves on photosynthesis, 70; 1-6 (2013)
30
G Noctor, S Veljovic Jovanovic, S Driscoll, L Novitskaya and CH Foyer, Annals of Botany, Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration?, 89; 841-850 (2002)
31
PH O'Farrell, J Biol Chem, High resolution two-dimensional electrophoresis of proteins, 250; 4007-4021 (1975)
32
YC Oh, OH Kang, JG Choi, YS Lee, OO Brice, HJ Jung, SH Hong, YM Lee, DW Shin and YS Kim, International Journal of Molecular Sciences, Anti-allergic activity of a platycodon root ethanol extract, 11; 2746-2758 (2010)
33
YS Park, Y Yoon and HS Ahn, World Journal of Gastroenterology, Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats, 13, 3493 (2007)
34
B Patwardhan, D Warude, P Pushpangadan and N Bhatt, Evidence-Based Complementary and Alternative Medicine, Ayurveda and traditional Chinese medicine: a comparative overview, 2; 465-473 (2005)
35
S Rehm and G Espig, CTA, The cultivated plants of the tropics and subtropics (1991)
36
KH Richau, F Kaschani, M Verdoes, TC Pansuriya, S Niessen, K Stüber, T Colby, HS Overkleeft, M Bogyo and RA Van der Hoorn, Plant Physiology, Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics, 158; 1583-1599 (2012)
37
L Rizhsky, H Liang and R Mittler, Plant Physiology, The combined effect of drought stress and heat shock on gene expression in tobacco, 130; 1143-1151 (2002)
38
AB Sachs, R Davis and R Kornberg, Molecular and Cellular Biology, A single domain of yeast poly (A)-binding protein is necessary and sufficient for RNA binding and cell viability, 7; 3268-3276 (1987)
39
G Sawicki and BI Jugdutt, Proteomics, Valsartan reverses posttranslational modifications of the ? subunit of ATP synthase during in vivo canine reperfused myocardial infarction, 7; 2100-2110 (2007)
40
A Tada, Y Kaneiwa, J Shoji and S Shibata, Chemical & pharmaceutical bulletin, Studies on the saponins of the root of Platycodon grandiflorum A. De Candolle. I. Isolation and the structure of platycodin-D, 23, 2965 (1975)
41
C Wang, GB Schuller Levis, EB Lee, WR Levis, DW Lee, BS Kim, SY Park and E Park, International immunopharmacology, Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-α in activated RAW 264.7 cells, 4; 1039-1049 (2004)
42
EM Williamson, Drug Safety, Drug interactions between herbal and prescription medicines, 26; 1075-1092 (2003)
43
H Zhao, J Sim, S Shim, Y Ha, S Kang and Y Kim, International Journal of Obesity, Antiobese and hypolipidemic effects of platycodin saponins in diet-induced obese rats: evidences for lipase inhibition and calorie intake restriction, 29; 983-990 (2005)
44
HL Zhao, KH Cho, YW Ha, TS Jeong, WS Lee and YS Kim, European journal of pharmacology, Cholesterol-lowering effect of platycodin D in hypercholesterolemic ICR mice, 537; 166-173 (2006)
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 60
  • No :1
  • Pages :97-106
  • Received Date : 2014-12-13
  • Accepted Date : 2015-02-28
  • DOI :https://doi.org/10.7740/kjcs.2014.60.1.097
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