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2026 Vol.71, Issue 2

Original Research Article

Breeding of Rice Stripe Virus (RSV)-Resistant Near-Isogenic Lines Using Rapid Generation Advance (RGA) and Marker-Assisted Backcrossing (MABC)
Seung-Kyo Jeong1
,
So-Myeong Lee2
,
Youngho Kwon2
,
Ju-Won Kang2
,
Sumin Jo2
,
Chanjeong Jeon3
,
Jin-Kyung Cha2
,
Hyeonjin Park2
,
Ye Rin An1
,
Ju-Young Choi4
,
Ji-Min Kim4
,
Woo-Jae Kim5
,
Jong-Min Jeong5
,
Jong-Hee Lee5*
1Post Master, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
2Junior Scientist, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
3Researcher, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
4Post Doctor, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
5Senior Scientist, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
*Corresponding Author
1 June 2026. pp. 39-47
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Abstract

The rice stripe virus (RSV) is a major constraint on japonica rice production in East Asia, and its distribution is expanding northward with climate warming. To develop RSV-resistant near-isogenic lines (NILs) for the susceptible cultivar, Odae, we integrated marker-assisted backcrossing (MABC) with a rapid generation advance (RGA) system. The resistant donor Haedamssal, which carries Stvb-i, was crossed with Odae, and foreground selection using the InDel7 marker and background selection with 116 Kompetitive Allele-Specific PCR (KASP) markers were performed. In the BC2F2 generation, the mean recurrent-parent genome recovery was 79.8%, and lines with a thousand-grain weight ≥25 g were selected. Agronomic and disease evaluations identified line YR33079-1-3, designated ‘Milyang397,’ which showed strong RSV resistance comparable to Haedamssal while retaining the agronomic and grain-quality traits of Odae. These results demonstrate that combining MABC with RGA enables the efficient development of high-recovery NILs within a shortened breeding cycle and provides a robust platform for validating and introgressing RSV resistance genes in early-maturing japonica rice.

Keywords
early-maturing cultivars
marker-assisted backcrossing (MABC)
near-Isogenic lines (NILs)
rice stripe virus (RSV)
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 71
  • No :2
  • Pages :39-47
  • Received Date : 2026-03-06
  • Revised Date : 2026-03-31
  • Accepted Date : 2026-04-01
  • DOI :https://doi.org/10.7740/kjcs.2026.71.2.039
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