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Original Research Article

Determination of Fatty Acid Composition in Peanut Seed by Near Infrared Reflectance Spectroscopy
Jeong Min Lee1
,
Suk-Bok Pae2†
,
Myoung-Gun Choung3
,
Myoung-Hee Lee2
,
Sung-Up Kim2
,
Eun-young Oh2
,
Ki-Won Oh2
,
Chan-Sik Jung2
,
In Seok Oh2
1Natural Products Research Team, National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea
2Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Korea
3Department of Herbal Medicine Resource, Kangwon National University, Samcheok 25913, Korea
*Corresponding Author
31 March 2016. pp. 64-69
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Abstract

This study was conducted to develop a fast and efficient screening method to determine the quantity of fatty acid in peanut oil for high oleate breeding program. A total of 329 peanut samples were used in this study, 227 of which were considered in the calibration equation development and 102 were utilized for validation, using near infrared reflectance spectroscopy (NIRS). The NIRS equations for all the seven fatty acids had low standard error of calibration (SEC) values, while high R2 values of 0.983 and 0.991 were obtained for oleic and linoleic acids, respectively in the calibration equation. Furthermore, the predicted means of the two main fatty acids in the calibration equation were very similar to the means based on gas chromatography (GC) analysis, ranging from 36.7 to 77.1% for oleic acid and 7.1 to 42.7% for linoleic acid. Based on the standard error of prediction (SEP), bias values, and R 2 statistics, the NIRS fatty acid equations were accurately predicted the concentrations of oleic and linoleic acids of the validation sample set. These results suggest that NIRS equations of oleic and linoleic acid can be used as a rapid mass screening method for fatty acid content analysis in peanut breeding program.

Keywords
Fatty acid
Linoleic acid
NIRS
Oleic acid
Peanut
References

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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 61
  • No :1
  • Pages :64-69
  • Received Date : 2015-07-17
  • Revised Date : 2016-03-11
  • Accepted Date : 2016-03-12
  • DOI :https://doi.org/10.7740/kjcs.2016.61.1.064
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