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2026 Vol.71, Issue 1 Preview Page

Original Research Article

Identification of Pre-harvest Sprouting Genotypes and Phenotypic Analysis of Germination Traits in Wheat

분자마커를 이용한 밀 수발아저항성 유전자형 및 발아특성 분석

Ye Rin An1
,
Jin-Kyung Cha2
,
Youngho Kwon2
,
So-Myeong Lee2
,
Seung-Kyo Jeong1
,
Woo-Jae Kim3
,
Hyeonjin Park2*
안 예린1
,
차 진경2
,
권 영호2
,
이 소명2
,
정 승교1
,
김 우재3
,
박 현진2*
1Post Master, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
2Junior Scientist, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
3Senior Scientist, Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Korea
1농촌진흥청 국립식량과학원 경지이용작물과 석사후연구원
2농촌진흥청 국립식량과학원 경지이용작물과 농업연구사
3농촌진흥청 국립식량과학원 경지이용작물과 농업연구관
*Corresponding Author
1 March 2026. pp. 13-23
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Abstract

Pre-harvest sprouting (PHS) is a major constraint affecting wheat grain quality under humid and unstable climatic conditions. This study evaluated PHS-related germination traits in 164 wheat (Triticum aestivum L.) cultivars and breeding lines, and examined the applicability of previously reported molecular markers for PHS-related breeding. Mean germination percentage (MGP), mean germination time (MGT), and mean germination speed (MGS) were measured using a controlled germination assay. Genotyping was performed using the KASP marker TaMFT_1617R, which targets a functional single nucleotide polymorphism in the promoter region of TaMFT-3A, a gene associated with seed dormancy. In addition, seed coat color-related variation was assessed using STS-Myb10 markers and phenol reaction-based scoring. The TaMFT-3A allele type showed a significant association with MGT, indicating differences in germination onset between allelic groups, whereas no significant differences were detected for MGP or MGS. In contrast, the Myb10 genotype and phenol-based seed coat color scores were significantly associated with MGS, but not with MGP or MGT. These results suggest that TaMFT-3A and seed coat color-related traits influence distinct components of germination behavior. Overall, the evaluated markers were not sufficient to reliably discriminate PHS tolerance based solely on germination traits under the experimental conditions. However, they provide useful supplementary information for early-stage selection when combined with phenotypic evaluation under PHS-inducing environments. This study highlights the importance of integrating molecular markers with environmental and phenotypic data for the effective selection of a PHS-tolerant wheat germplasm.

Keywords
wheat
pre-harvest sprouting
seed dormancy
marker selection
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 71
  • No :1
  • Pages :13-23
  • Received Date : 2026-01-19
  • Revised Date : 2026-02-04
  • Accepted Date : 2026-02-23
  • DOI :https://doi.org/10.7740/kjcs.2026.71.1.013
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