All Issue

2014 Vol.59, Issue 2 Preview Page

Original Research Article

EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)
Yong-Hwa Lee1*
,
Kwang-Soo Kim1
,
Young-Seok Jang1
,
In-Hu Choi1
1Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration, Muan 533-834, Korea
*Corresponding Author
30 June 2014. pp. 128-133
PDF XML Citation
Abstract

Rapeseed (Brassica napus L.) oil with high oleic acid content is of great interest for both food and non-food uses. The ‘Tamla’ variety, characterized by oleic acid content of approximately 69%, was treated with 1% ethyl methane sulfonate (EMS) to induce mutations in the fatty acid biosynthesis pathway. M1 plants were selfed and subsequent generations (M2, M3, and M4 mutants) were analyzed to identify mutants having increased levels of oleic acid. M2 mutants showed oleic acid content ranging from 13.5% to 76.9% with some mutants (TR-458 and TR-544) having up to 74.7% and 76.9% oleic acid, which was an increase of nearly 5% and 7%, respectively, compared to untreated cv ‘Tamla’. We selected two M3 mutants with >75% oleic acid content. One mutant (TR-458-2) had increased oleic acid (75.9%) and decreased linoleic acid (12.5%) and linolenic acid (4.4%) contents. The other (TR-544-1) showed increased oleic acid content (75.7%) and decreased linoleic acid (13.5%) and linolenic acid (3.3%) contents. The accumulation or reduction of oleic acid content in the selected M4 mutants was also accompanied by a simultaneous decrease or increase in linoleic and linolenic acid contents. The high-oleic lines could be utilized further in breeding programs for improvement of rapeseed oil quality.

Keywords
rapeseed
mutagens
EMS
oleic acid
fatty acid
References

REFERENCES

1
DL Auld, MK Heikkinen, DA Erickson, JL Sernyk and JE Romero, Crop Science, Rapeseed mutants with reduced levels of polyunsaturated fatty acids and increased levels of oleic acid, 32(3); 657-662 (1992)
2
A Bühsenschüz-Nothdurft, A Schuster and W Friedt, Industrial Crops and Products, Breeding for modified fatty acid composition via experimental mutagenesis in Camelina sativa (L.) Crtz, 7(2); 291-295 (1998)
3
BM Craig, Canadian Journal of Plant Science, Varietal and environmental effects on rapeseed. 3. Fatty acid composition of 1958 varietal tests, 41; 204-210 (1961)
4
M Jacobs, Radiation Botany, Comparison de I'action mutagenique d’agents alkylants et des radiations gamina chez Arabidopsis thaliana, 9; 251-268 (1969)
5
DW James and HK Dooner, Theoretical and Applied Genetics, Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition, 80(2); 241-245 (1990)
6
R Jönsson, Hereditas, Erucic-acid heredity in rapeseed Brassica napus L. and Brassica campestris, 86(2); 159-170 (1977)
7
YS Jang, KS Kim, YH Lee, HJ Cho and SJ Suh, Journal of Plant Biotechnology, Review of property and utilization of oil crop for biodiesel, 37(1); 25-46 (2010)
8
YH Lee, KS Kim, YS Jang, HJ Cho, SS Nam and SJ Suh, The Journal of the Korean Society of International Agriculture, Breeding of F1 hybrid rapeseed and the cultivation for biodiesel production in Korea, 22(4); 341-345 (2010)
9
A Patil, SP Taware, MD Oak, SA Tamhankar and VS Rao, Journal of the American Oil Chemists' Society, Improvement of oil quality in soybean [Glycine max (L.) Merrill] by mutation breeding, 84(12); 1117-1124 (2007)
10
SM Rahman, Y Takagi, K Kubota, K Miyamoto and T Kawakita, Bioscience, Biotechnology, and Biochemistry, High oleic acid mutant in soybean induced by x-ray irradiation, 58(6); 1070-1072 (1994)
11
G Rakow and DI McGregor, Journal of the American Oil Chemists' Society, Opportunities and problems in modification of levels of rapeseed C18 unsaturated fatty acids, 50(10); 400-403 (1973)
12
G Röbbelen and K Kräling, Industrial Crops and Products, Rapeseed oils high in single fatty acid contents for oleochemical uses, 1; 303-309 (1993)
13
KD Savant and VS Kothekar, Journal of Phytology, Induction of variability in fatty acid profile in sesame (Sesamum indicum L.), 3(12); 1-3 (2011)
14
FA Sheikh, B Lone, S Najeeb, AB Shikari, GA Parray, RR Rather and RS Khudwani, Journal of Agricultural and Biological Science, Induced mutagenesis for seed quality traits in Ethiopian mustard (Brassica carinata A. Braun), 4(2); 42-46 (2009)
15
S Spasibionek, Plant Breeding, New mutants of winter rapeseed (Brassica napus L.) with changed fatty acid composition, 125(3); 259-267 (2006)
16
L Velasco, JM Fernández-Martínez and A De Haro, Canadian Journal of Plant Science, Induced variability for C18 unsaturated fatty acids in Ethiopian mustard, 77(1); 91-95 (1997)
17
J Zhao, Z Dimov, HC Becker, W Ecke and C Mölers, Molecular Breeding, Mapping QTL controlling fatty acid composition in a doubled haploid rapeseed population segregating for oil content, 21(1); 115-125 (2008)
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 59
  • No :2
  • Pages :128-133
  • Received Date : 2013-07-24
  • Revised Date : 2014-01-20
  • Accepted Date : 2014-03-10
  • DOI :https://doi.org/10.7740/kjcs.2014.59.2.128
Journal Informaiton The Korean Journal of Crop Science The Korean Journal of Crop Science
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close