All Issue

2015 Vol.60, Issue 4 Preview Page

Original Research Article

Evaluation of Cytotoxicity, Carbohydrate, and Lipid Inhibitory Activity of Codonopsis lanceolata using Different Solvent Fractions
Hee-Ock Boo1
,
Jeong-Hun Park1
,
Seung-Mi Kim1
,
Sun-Hee Woo2
,
Hyeon-Yong Park*†
1Department of Biology, College of Natural Science, Chosun University, Gwangju 61452, Korea
2Department of Crop Science, Chungbuk National University, Cheongju 28644, Korea
*Corresponding Author
31 December 2015. pp. 498-503
PDF XML Citation
Abstract

This study was conducted to evaluate the cytotoxicity and α-amylase, α-glucosidase, pancreatic lipase inhibition in vitro by different solvent fractions from the roots of Codonopsis lanceolata. The values of IC50 against Calu-6 cell showed a high effect in n-hexane fraction (10.13 μg/mL) whereas DW fraction exhibited the weakest inhibition on cell viability, having an IC50 value of over 1,000 μg/mL. The values of IC50 against HCT-116 cell showed the highest activity in n-BuOH fraction (102.01 μg/mL), followed by n-hexane fraction (145.85 μg/mL), methylene chloride fraction (332.02 μg/mL), ethyl acetate fraction (462.93 μg/mL) and DW fracion (>1,000 μg/mL). α-Amylase inhibitory activity in methylene chloride fraction and ethyl acetate fraction was found to have a higher inhibitory effect with 24.5% and 25.6% than the other fractions. The highest α-glucosidase inhibitory activity was observed from the ethyl acetate fraction extract, while the extract of DW fraction showed the lowest level of inhibitory activity at given experiment concentration. The pancreatic lipase inhibitory activity of C. lanceolata was found to have a higher the effect in ethyl acetate fraction. Inhibition of lipase activity of the ethyl acetate fraction and n-hexane fraction showed a relatively high, while the extract of DW fraction showed the lowest level at given experiment concentration. These results suggested that the roots of C. lanceolata may assist in the potential biological activity on carbohydrate, lipid Inhibitory activity and anticancer activity.

Keywords
Cytotoxicity
α-Amylase
α-Glucosidase
Pancreatic lipase
Codonopsis lanceolata
References

REFERENCES

1
M H Davidson, J Hauptman, M DiGirolamo, J P Foreyt, C H Halsted and D Heber, JAMA, Weight control and risk factor reduction in obese subjects treated for 2 years with orlistat: A Randomized Controlled Trial, 281; 235-242 (1999)
2
S Frantz, L Calvillo, J Tillmanns, I Elbing, C Dienesch, H Bischoff, G Ertl and J Bauersachs, FASEB J, Repetitive postprandial hyperglycemia increases cardiac ischemia/reperfusion injury: prevention by the alpha-glucosidase inhibitor acarbose, 19; 591-593 (2005)
3
Y Hara and M Honda, Agri. Biol. Chem, The inhibition of a-amylase by tea polypphenols, 54(8); 1939-1945 (1990)
4
Q He, Y Lv and K Yao, Food Chem, Effects of tea polyphenols on the activities of a-amylase, pepsin, trypsin and lipase, 101; 1178-1182 (2006)
5
E H Joh, I A Lee, S J Han, S J Chae and D H Kim, Int. J. Colorectal Dis, Lancemaside A ameliorates colitis by inhibiting NF-jB activation in TNBS-induced colitis mice, 25; 545-551 (2010)
6
S W Jung, A J Han, H J Hong, M G Choung, K S Kim and S H Park, Agric. Chem. Biotechnol, α-Glucosidase inhibitors from the roots of Codonopsis lanceolata Trautv, 49(4); 162-164 (2006)
7
H Y Kim, S H Lim, Y H Park, H J Ham, K J Lee, D S Park, K H Kim and S M Kim, J. Korean Soc. Food Sci. Nutr, Screening of a-amylase, a-glucosidase and lipase inhibitory activity with Gangwon-do wild plants extracts, 40(2); 308-315 (2011)
8
Y M Kim, Y K Jeong, M H Wang, W Y Lee and H I Rhee, Nutrition, Inhibitory effect of pine extract on a-glucosidase activity and postprandial hyperglycemia, 21; 756-761 (2005)
9
Y Kimura, Y Araki, A Takenaka and K Igarashi, Biosci. Biotechnol. Biochem, Protective effect of dietary nasunin and parapect induced oxidative stress in rat, 63; 799-804 (2006)
10
E Kintzios, Crit. Rev. Plant Sci, Terrestrial plant-derived anticancer agents and plant species used in anticancer research, 25; 79-113 (2006)
11
H Kurihara, H Shibata, Y Fukui, Y Kiso, J K Xu, X S Yao and H Fukami, J. Agric. Food Chem, Evaluation of the hypolipemic property of Camellia sinensis var. ptilophylla on postprandial hypertriglyceridemia, 54; 4977-4981 (2006)
12
J J Marshall and C M Lauda, J Biol Chem, Purification and properties of phaseolamin, an inhibitor of alpha-amylase, from the kidney bean, Phaseolus vulgaris, 250; 8030-8037 (1975)
13
N P Moller, N Roos and J Schrezenmeir, Phytother. Res, Lipase inhibitory activity in alcohol extracts of worldwide occurring plants and propolis, 23; 585-586 (2009)
14
S J Oh, J H Lee, K S Ko, D B Shin and S C Koh, Korean J. Plant Res, Antioxidative activity, including inhibitory activities of ACE, APN and a-amylase, in Theaceae plants native to Jeju Island, 23(5); 406-414 (2010)
15
H J Park, M J Kim, E Ha and JH Chung, Phytomedicine, Apoptotic effect of hesperidin through caspase 3 activation in human colon cancer cells, SNU-C4, 15; 147-151 (2008)
16
B B Rahul and K B Kamlesh, Drug Discov. Today, Pancreatic lipase inhibitors from natural sources: unexplored potential, 12; 879-889 (2007)
17
T Satoyama, T Hara, M Murata and Y Fujio, Nippon Nogeikagaku Kaishi, A simple assay method for a-amylase using microplates, 72; 933-936 (1998)
18
N Sharma, V K Sharma and S Y Seo, J. Ethnopharmacol, Screening of some medicinal plants for anti-lipase activity, 97; 453-456 (2005)
19
R Vadivelan, S P Dhanabal, A Wadhawani and K Elango, IJPSR, a-Glucosidase and a-amylase inhibitory activities of Raphanus sativus Linn, 3 (9); 3186-3188 (2012)
20
S Vijayarathna and S Sasidharan, Asian Pac. J. Trop Biomed, Cytotoxicity of methanolextracts of Elaeis guineensis on MCF-7 and Vero cell lines, 2(10); 826-829 (2012)
21
H Z Wang, C H Chang, C P Lin and M C Tsai, J. OcularPharm Therapeutics, UsingMTT viability assay to test the cytotoxicity of antibiotics and steroid to cultured porcine corneal endothelial cells, 12; 35-43 (2006)
22
L Wang, M L Xu, J H u, S K Rasmussen and M H Wang, Food Chem. toxicol, Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells – Involvement of ROS generation and polyamine depletion, 49; 149-154 (2011)
23
H Yan-Wei, L Chun-Yu, D Chong-Min, W Wen-Qian and G Zhen-Lun, J. Ethnopharmacol, Induction of apoptosis in human hepatocarcinoma SMMC-7721 cells in vitro by flavonoids from Astragalus complanatus, 123; 293-301 (2009)
24
J Zhang, M J Kang, M J Kim, M E Kim, J H Song, Y M ee and J I Kim, Nutr. Res. Pract, Pancreatic lipase inhibitory activity of taraxacum officinale in vitro and in vivo, 2(4); 200-203 (2008)
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 60
  • No :4
  • Pages :498-503
  • Received Date : 2015-12-07
  • Accepted Date : 2015-12-10
  • DOI :https://doi.org/10.7740/kjcs.2015.60.4.498
Journal Informaiton The Korean Journal of Crop Science The Korean Journal of Crop Science
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close