Growth Characteristics and Comparative Proteome Analysis of Adzuki Bean Leaves at the Early Vegetative Stage under Waterlogging Stress

Hae-Ryong Jeong; Soo-Jeong Kwon; Sung-Hyun Yun; Min-Young Park; Hee-Ock Boo; Hag-Hyun Kim; Moon-Soon Lee; Sun-Hee Woo

doi:10.7740/kjcs.2022.67.4.211

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2022 Vol.67, Issue 4 Preview Page Next Page

Original Research Article

Growth Characteristics and Comparative Proteome Analysis of Adzuki Bean Leaves at the Early Vegetative Stage under Waterlogging Stress 논 토양 조건에서 팥 유묘기의 생육특성과 단백질 발현 양상

1 December 2022. pp. 211-221

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Abstract

Recently, the demand for the cultivation of upland soil has been increasing, and the rate of conversion of paddy soil into upland soil is also increasing. Theincrease in uneven precipitation due to climate change has resulted in dramatic effects of waterlogging stress on upland crops. Therefore, the present study was conducted to investigate the changes in growth characteristics and the expression patterns of proteins at the two-leaf stage of adzuki beans. The domestic cultivar, Arari (Miryang No. 8), was used to test waterlogging stress. At the two-leaf stage of adzuki beans, plant height slightly decreased androot fresh weight showed significant changes after 3 days of waterlogging treatment. Chlorophyll content was also significantly different after 3 days of waterlogging treatment compared to its content in control plants. Using two-dimensional gel electrophoresis, more than 400 protein spots were identified. Twenty-one differentially expressed proteins from the two-leaf stage were analyzed using linear trap quadrupole–Fourier transform–ion cyclotron resonance mass spectrometry. Of these 21 proteins, 9 were up-regulated and 12 were down-regulated under waterlogging treatment. Protein information resource (https://pir.georgetown.edu/) categories were assigned to all 49 proteins according to their molecular function, cellular component localization, and biological processes. Most of the proteins were found to be involved in the biological process, carbohydrate metabolism and were localized in chloroplasts.

Keywords

adzuki bean

growth characteristics

proteome

vegetative stage

waterlogging stress

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Information

Publisher :The Korean Society of Crop Science
Publisher(Ko) :한국작물학회
Journal Title :The Korean Journal of Crop Science
Journal Title(Ko) :한국작물학회지
Volume : 67
No :4
Pages :211-221
Received Date : 2022-10-24
Revised Date : 2022-11-22
Accepted Date : 2022-11-22
DOI :https://doi.org/10.7740/kjcs.2022.67.4.211

The Korean Journal of Crop Science ISSN:0252-9777(Print) 2287-8432(Online) 한국작물학회지

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