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2021 Vol.66, Issue 3 Preview Page

Original Research Article

Expression Analysis of Anthocyanin Biosynthetic Genes of Tassel and Silks in Gwangpyeongok and Dacheongok

광평옥과 다청옥의 수이삭과 수염에서 안토시아닌 생합성 유전자 발현 분석

Young Sam Go1*
,
Hwan Hee Bae1
,
Yu Chan Choi1
,
Jae Han Son1
,
Jun Young Ha2
,
Seong Hyu Shin3
,
Tae Wook Jung3
고 영삼1*
,
배 환희1
,
최 유찬1
,
손 재한1
,
하 준영2
,
신 성휴3
,
정 태욱3
1Agriculture Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
2Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
3Agriculture Senior Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
1농촌진흥청 국립식량과학원 중부작물부 농업연구사
2농촌진흥청 국립식량과학원 중부작물부 연구원
3농촌진흥청 국립식량과학원 중부작물부 농업연구관
*Corresponding Author
1 September 2021. pp. 240-247
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Abstract

Anthocyanins are known to be involved in various functions such as antioxidant and antibacterial activities in plants. Although studies on anthocyanins in corn have been conducted recently, basic research related to anthocyanin biosynthesis is insufficient. In this study, we examined the molecular biological and physicochemical properties related to anthocyanin biosynthesis in the tassel and silks of Gwangpyeongok and Dacheongok cultivars. Anthocyanins were not synthesized in either the tassel or silks in Gwangpyeongok, whereas were synthesized in both in Dacheongok. The total anthocyanin content was approximately 30 times higher in the tassel and silks of Dacheongok than in those of Gwangpyeongok. In addition, C-3-G was measured only in the tassel of Dacheongok, and C-3-G, Pg-3-G, and M-3-G were 45.2 times, 27.3 times, and 37.6 times higher, respectively, in the silks of Dacheongok than of Gwangpyeongok. Expression of F3'H, DFR, and GST genes decreased in the tassel, and that of F3'H and DFR genes decreased in the silks of Gwangpyeongok. It was further confirmed that transcription factor P1 and R1 regulate the expression of anthocyanin biosynthetic genes in the tassel and silks, respectively, in Gwangpyeongok. Linoleic acid (C18:2) decreased by 6.6% and 10.9%, and linolenic acid (C18:3) increased by 8.5% and 8.5%, in the tassel and silks, respectively, of Gwangpyeongok compared to those of Dacheongok. Palmitic acid (C16:0) increased by 4.1% and oleic acid (C18:1) decreased by 2.1% in the silks of Gwangpyeongok compared to that in Dacheongok. In addition, the total fatty acid content in the tassel and silks increased by 10.3% and 30.4%, respectively, in Gwangpyeongok compared to that in Dacheongok. However, no significant results were observed in the analysis of phytosterol components. These results may be utilized as useful resources for the development of functional corn containing a large amount of anthocyanins.

Keywords
anthocyanin
corn
fatty acid
gene expression
phytosterol
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 66
  • No :3
  • Pages :240-247
  • Received Date : 2021-04-09
  • Revised Date : 2021-07-06
  • Accepted Date : 2021-07-19
  • DOI :https://doi.org/10.7740/kjcs.2021.66.3.240
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