Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat

Seong-Woo Cho; Soo-Jeong Kwon; Swapan Kumar Roy; Sun-Hee Woo

doi:10.7740/kjcs.2014.60.1.029

All Issue

2015 Vol.60, Issue 1 Preview Page Next Page

Original Research Article

Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat

31 March 2015. pp. 29-32

PDF XML

Abstract

Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

Keywords

Buckwheat

2-DE

Efficient Separation Method

Seed Storage Protein

Proteome Approach

References

REFERENCES

B Dolinsek, Proceeding of 1st Buckwheat Symp, Studies of polymorphism of electrophoretic proteins in buckwheatLjubljana; 61-68 (1980)

Z Görg, Electrophoresis, Two-dimensional polyacrylamide gel electrophoresis, with immobilized pH gradients in the first dimension, of barley seed proteins: Discrimination of cultivars with different malting grades, 13, 192 (1992)

UK Laemmli, Nature, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, 227; 680-685 (1970)

A Nair and T Adachi, Plant Biotech, Immunodetection and characterization of allergenic proteins in common buckwheat (Fagopyrum esculentum), 16; 219-224 (1999)

K Nishiyama, S Lachmann and M Miura, Proceed. of ICOBB, Electrophoretic property of buckwheat seed proteinsMiyazaki; 215-222 (1991)

PH O' Farrell, J. Biol. Chem, High resolution two-dimensional electrophoresis of proteins, 250; 4007-4021 (1975)

O Ohnishi, Fagopyrum, Search for the wild ancestor of buckwheat. I. Description of new Fagopyrum (Polygonaceae) species and their distribution in China and Himalayan hills, 15; 18-28 (1998)

A Pandy and M Mann, Nature, Proteomics to study genes and genomes, 405; 837-846 (2000)

TE Shevchuk, Fagopyrum, The polymorphism of buckwheat 13s globulin, 6, 9 (1986)

S Svetek, Porch. of IPBA. Rogla, Electrophoretic analysis of buckwheat (Fagopyrum esculentum Moench) seed proteins; 161-170 (1994)

RR Svetlana, VR Maksimovic and EI Varkonji-Gasic, J. Agric. Food. Chem, Characterization of Buckwheat Seed Storage Proteins, 44; 972-974 (1996)

I Tahir and T Farooq, Fagopyrum, Review article on buckwheat, 8; 33-53 (1988)

A Urisu, Y Kondo, Y Morita, E Wada, M Tsuruta, T Yasaki, K Yamada, H Kuzaya, M Suzuki, K Titani and K Kurosawa, Allerg. Clin. Immunol, Identification of a major allergen of buckwheat seeds by immunoblotting methods, 6; 151-155 (1994)

SH Woo, M Fukuda, N Islam, M Takaoka, H Kawasaki and H Hirano, Electrophoresis, Efficient peptide mapping and its application to identification of embryo proteins in the rice proteome analysis, 23; 647-654 (2002)

Information

Publisher :The Korean Society of Crop Science
Publisher(Ko) :한국작물학회
Journal Title :The Korean Journal of Crop Science
Journal Title(Ko) :한국작물학회지
Volume : 60
No :1
Pages :29-32
Received Date : 2014-12-26
Accepted Date : 2015-02-09
DOI :https://doi.org/10.7740/kjcs.2014.60.1.029

The Korean Journal of Crop Science ISSN:0252-9777(Print) 2287-8432(Online) 한국작물학회지

All Issue

REFERENCES