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2017 Vol.62, Issue 3 Preview Page

Original Research Article

Effects of Medium Compositions and Plant Growth Regulators on in vitro Organogenesis in Cultured Explants of Platycodon grandiflorum Species
Soo Jeong Kwon1
,
Swapan Kumar Roy2
,
Hye-Rim Kim2
,
Young-Ja Moon1
,
Ki-Hong Yoon3
,
Sun Hee Woo2
,
Hee Ock Boo4
,
Jin-Woog Koo5
,
Hag Hyun Kim1*
1Dept. of Food Nutrition and Cookery, Woosong College, Daejeon 34606, Korea
2Dept. of Crop Science, Chungbuk National University, Cheong-ju 28644, Korea
3Dept. of Food Science and Biotechnology, Woosong University, Daejeon 34606, Korea
4AGROLEAD Co, Ltd., 995-24 1do 2-dong, Jeju City 63265, Korea
5NOTHING dESIGN GROUP [GWANGJU], Gwangsan-gu, Gwangju 62224, Korea
*Corresponding author: Hag Hyun Kim; +82-42-629-6988; (hkyushu@hanmail.net)
30 September 2017. pp. 259-274
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Abstract

Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on ¼ MS for P. grandiflorum with wild and green petals and on ⅛ MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on ¼ MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, ¼ MS supplemented with 1 mg L-1 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on 0.5 mg ․ L-1 thidiazuron (TDZ) from double petal explants grown on ⅛ MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 62
  • No :3
  • Pages :259-274
  • Received Date : 2017-08-12
  • Revised Date : 2017-09-04
  • Accepted Date : 2017-09-07
  • DOI :https://doi.org/10.7740/kjcs.2017.62.3.259
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