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2019 Vol.64, Issue 4 Preview Page

Original Research Article

Validation of a Method and Evaluation of Antioxidant Activity for the Simultaneous Determination of Riboflavin and Coixol in Coix lacryma-jobi var. ma-yuen Stapf Sprouts

율무 새싹 추출물의 Riboflavin과 Coixol의 동시 분석법 검증 및 항산화 활성

Ji Yeon Lee1
,
Jung Yong Park1
,
Chun-Geon Park2
,
Dong Hwi Kim2
,
Yun-Jeong Ji3
,
Su Ji Choi2
,
MyeongWon Oh3
,
Hosop Hwang1
,
Yunji Lee4
,
Jintae Jeong4
,
Jeong Hoon Lee4
,
Kyung Hye Seo4*
이 지연1
,
박 정용1
,
박 춘근2
,
김 동휘2
,
지 윤정3
,
최 수지2
,
오 명원3
,
황 호섭1
,
이 윤지4
,
정 진태4
,
이 정훈4
,
서 경혜4*
1Student, Department of Food Science and Biotechnology, Chungbuk National University
2Senior Researcher, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science
3Posdoc, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science
4Researcher, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science
1충북대학교 식품공학과 대학원생
2농촌진흥청 인삼특작부 연구관
3농촌진흥청 인삼특작부 박사후연구원
4농촌진흥청 인삼특작부 연구사
* Corresponding Author
31 December 2019. pp. 452-458
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Abstract

Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography–diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 °C under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC50], 68.9 ± 4.1 g/mL; and ABTS, IC50, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.

Keywords
bioactive compounds
Coix lacryma-jobi
correlation analysis
free radical scavenging
standardization
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 64
  • No :4
  • Pages :452-458
  • Received Date : 2019-08-10
  • Revised Date : 2019-10-21
  • Accepted Date : 2019-10-24
  • DOI :https://doi.org/10.7740/kjcs.2019.64.4.452
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