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2020 Vol.65, Issue 2 Preview Page

Original Research Article

Mitigation Effects of Foliar-Applied Hydrogen Peroxide on Drought Stress in Sorghum bicolor

과산화수소 엽면 처리에 의한 수수에서 한발 스트레스 완화 효과

Doo-Do Shim1*
,
Seung-Ha Lee2*
,
Jong-Il Chung3
,
Min Chul Kim3
,
Jung-Sung Chung3
,
Yeong-Hun Lee2
,
Seung-Ho Jeon4
,
Gi-Eun Song5
,
Sang-In Shim3**
심 두보1*
,
이 승하2*
,
정 종일3
,
김 민철3
,
정 정성3
,
이 영훈2
,
전 승호4
,
송 기은5
,
심 상인3**
1Researcher, Institute of Hadong Green Tea, 3748-14, Seomjingang-daero, Hwagae-myeon, Hadong-gun 52304, Korea
2MSc Student, Department of Agronomy, Gyeongsang National University, Jinju 52828, Korea
3Professor, Department of Agronomy, Gyeongsang National University, Jinju 52828, Korea
4Professor, Wellness Resources Department, Sunchon National University, Suncheon-si 57922, Korea
5PhD Student, Department of Applied Biological Science, Applied Biology (BK Plus), Gyeongsang National University, Jinju 52828, Korea
1(재)하동녹차연구소 연구원
2경상대학교 농학과 석사과정
3경상대학교 농학과 교수
4순천대학교 웰빙자원학과 교수
5경상대학교 응용생명과학부 BK21+ 프로그램 박사과정
*These authors contributed equally.
**Corresponding Author
1 June 2020. pp. 113-123
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Abstract

Global climatic change and increasing climatic instability threaten crop productivity. Due to climatic change, drought stress is occurring more frequently in crop fields. In this study, we investigated the effect of treatment with hydrogen peroxide (H2O2) before leaf development on the growth and yield of sorghum for minimizing the damage of crops to drought. To assess the effect of H2O2 on the growth of sorghum plant, 10 mM H2O2 was used to treat sorghum leaves at the 3-leaf stage during growth in field conditions. Plant height, stem diameter, leaf length, and leaf width were increased by 7.6%, 9.6%, 8.3% and 11.5%, respectively. SPAD value, chlorophyll fluorescence (Fv/Fm), photosynthetic rate, stomatal conductance, and transpiration rate were increased by 3.0%, 4.9%, 26.0%, 23.4% and 12.7%, respectively. The amount of H2O2 in the leaf tissue of sorghum plant treated with 10 mM H2O2 was 0.7% of the applied amount after 1 hour. The level increased to approximately 1.0% after 6 hours. The highest antioxidant activity measured by the Oxygen Radical Absorbance Capacity assay was 847.3 μmol·g-1 at 6 hour after treatment. However, in the well-watered condition, the concentration of H2O2 in the plant treated by the foliar application of H2O2 was 227.8 μmol·g-1 higher than that of the untreated control. H2O2 treatment improved all the yield components and yield-related factors. Panicle length, plant dry weight, panicle weight, seed weight per plant, seed weight per unit area, and thousand seed weight were increased by 8.8%, 18.0%, 24.4%, 24.7%, 29.9% and 7.1%, respectively. Proteomic analysis showed that H2O2 treatment in sorghum increased the tolerance to drought stress and maintained growth and yield by ameliorating oxidative stress.

Keywords
drought stress
H2O2
oxidative stress
proteomics
sorghum
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :2
  • Pages :113-123
  • Received Date : 2020-03-17
  • Revised Date : 2020-04-08
  • Accepted Date : 2020-05-11
  • DOI :https://doi.org/10.7740/kjcs.2020.65.2.113
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