All Issue

2016 Vol.61, Issue 3 Preview Page

Original Research Article

옥수수수염 추출물의 항산화효과 및 피부각질세포 보호효과

Antioxidative and Protective Effects of Corn Silk (Zea mays L.) Extract on Human HaCaT Keratinocyte

Hyun Young Kim1
,
Woo Duck Seo1
,
Kyung Hye Seo2
,
Mi-Ja Lee1
,
Sik-Won Choi1
,
Kwang-Sik Lee1
,
Sun Lim Kim1
,
Hyeon Jung Kang1*
김 현영1
,
서 우덕1
,
서 경혜2
,
이 미자1
,
최 식원1
,
이 광식1
,
김 선림1
,
강 현중1*
1Crop Foundation Division, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju-gun, 55365, Korea
2Department of Herbal Crop Research, NIHHS, RDA, Eumseong-gun, 27709, Korea
1농촌진흥청 국립식량과학원 작물기초기반과
2농촌진흥청 원예특작과학원 인삼특작부
*Corresponding Author
30 September 2016. pp. 184-190
PDF XML Citation
Abstract

We investigated the antioxidative and protective effects of corn silk (Zea mays L.) ethanol extracts on human HaCaT cells and erythrocytes. The NICS-2 fraction, extracted from corn silk, exhibited favorable 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activities with IC50valuesof13.3±0.3 μg/mL and 14.2 ± 0.1 μg/mL when compared with those of α-tocopherol, a positive control, with IC50=10.4± 02.2 and 22.2 ± 3.6 μg/mL, respectively. In addition, we investigated skin protection effects of NICS extracts of corn silk in HaCaT keratinocytes. To investigate the pharmacological potential of NICS-1 and NICS-2 extracts of corn silk on UV-B-induced damage in HaCaT cells, we measured the activity of interleukin (IL) 1a. Our results showed that all the corn silk extracts inhibited the UV-B-induced activity of IL-1a. In particular, NICS-1 extracts of corn silk significantly suppressed IL-1a activity in a dose-dependent manner without inducing cytotoxicity. These results indicate that the ethanol extracts of corn silk (Zea mays L.) could function as natural cytoprotective agents and antioxidants in biological systems, particularly the skin exposed to UV radiation, by protecting cellular membrane against reactive oxygen species (ROS).

Keywords
antioxidant
corn silk
cytoprotective effect
HaCaT keratinocyte cells
References

References

1
F Afaq, V M Adhami, N Ahmad and H Mukhtar, Front. Biosci, Botanical antioxidants for chemoprevention of photocarcinogenesis, 7; 784-792 (2002)
2
S M Cha, B Y Son, J S Lee, S B Baek, S L Kim, J H Ku, J J Hwang, B H Song, S H Woo, Y U Kwon and J T Kim, Korean J. Crop Sci, Effect of Particle Size on Physico-Chemical Properties and Antioxidant Activity of Corn Silk Powder, 57(1); 41-50 (2012)
3
G J Clydesdale, G W Dandie and H K Muller, Immunol. Cell Biol, Ultraviolet light induced injury: Immunological and inflammatory effests, 79; 547-568 (2001)
4
N Di Girolamo, D Wakefield and M T Coroneo, Invest. Ophth. Vis. Sci, UV-B mediated induction of cytokines and growth factors in pterygium epithelial cells involves cell surface receptors and intracellular signaling, 47; 2430-2437 (2006)
5
M ErdenInal, A Kahraman and T Ken, Exp. Dermatol, Beneficial effects of quercetin on oxidative stress induced by ultraviolet Clin, 26; 536-539 (2001)
6
G J Fisher, Z Q Wang, S C Datta, J Varani, S Kang and J J Voorhees, New Engl. J. Med, Pathophysiology of premature skin aging induced by ultraviolet light, 337; 1419-1428 (1997)
7
T Fossen, R Slimestad and O M Andersen, J. Agric. Food Chem, Anthocyanins from maize (Zea mays) and reed canarygrass (Phalaris arundinacea), 49; 2318-2321 (2001)
8
B A Gilchrest, Dermatol. Nurs, Skin aging and photoaging, 2; 79-82 (1990)
9
T Y Ha, Korean J. Crop Sci, Development of functional food materials for healthy life, 51; 26-39 (2006)
10
Y S Han and E S Jung, Korean Aesthetic. Soc, A study of correlation between antioxidant activity and whitening effect of plant extract, 1; 11-22 (2003)
11
E Y Hwang, Y H Kong, Y C Lee, Y C Kim, K M Yoo, Y O Jo and S Y Choi, J. Ginseng Res, Comparison of phenolic compound contents between white and red ginseng and their inhibitory effect on melanin biosynthesis, 30; 82-87 (2006)
12
M K Kang, S K Kim and Y H Kang, Korean J. Plant Res, Antioxidant Activity and Functional Components of Corn Silk (Zea mays L), 22(4); 323-329 (2009)
13
S K Katiyar, M S Matsui, C A Elmets and H Mukhtar, Photochem. Photobiol, Polyphenolic antioxidant(-)-epigallocatechin-3-gallate from green tea reduces UV-B-induced inflammatory responses and infiltration of leukocytes in human skin, 69; 148-153 (1999)
14
H I Kim, M J Oh, J S Kim, S C Lee, J Kwon and C H Lee, J Physiol. & Pathol. Korean Med, Protective Effect of Fermented Red Ginseng Extracts on Photoaging Skin of Induced by UV-B in Hairless Mice, 29(1); 58-65 (2015)
15
S L Kim, M E Snook and J O Lee, Korean J. Crop Sci, Radical Scavenging Activity and Cytotoxicity of Maysin (C-glycosylflavone) isolated from Silks of Zea mays L, 48; 392-396 (2003)
16
S L Kim, M E Snook, E H Kim and C H Park, Korean J. Crop Sci, Identification of maysin and related flavonoid analogues in corn silks, 45(3); 151-157 (2000)
17
E A Lee, P F Byrne, M D McMullen, M E Snook, B R Wiseman, N W Widstrom and E H Coe, Genetics, Genetic mechanisms underlying apimaysin and maysin synthesis and corn earworm antibiosis in maize (Zea mays L), 149; 1997-2006 (1998)
18
T Osawa, I Uritani, VV Garcia and EM Mendoza, Postharvest Biochemistry of Plank Food Material in the TropicsNovel natural antioxidant for utilization in food and biological system; 241-251, Tokyo, Japan. Japan Scientific Societies Press. (1994)
19
S J Rhie, J. Kor. Soc. Cosm, The effect of lavender oil on the activity of antioxidant enzymes to ultraviolet-damaged skin, 13; 467-474 (2007)
20
T Shimauchi, K Sugita, D Nishio, H Isoda, S Abe, Y Yamada, R Hino, M Ogata, K Kabashima and Y Tokura, J. Dermatol. Sci, Alterations of serum Th1 and Th2 chemokines by combination therapy of interferon-? and narrowband UV-B in patients with mycosis fungoides, 50; 217-225 (2008)
21
K Tanaka, J Hasegawa, K Asamitsu and T Okamoto, J. Pharmacol. Exp. Ther, Prevention of the ultraviolet B-mediated skin photoaging by a nuclear factor ?B inhibitor, parthenolide, 315; 624-630 (2005)
22
D Yarosh, D Both, J Kibitel, C Anderson, C Elmets, D Brash and D Brown, Photodermatol. Photo, Regulation of TNFa production and release in human and mouse keratinocytes and mouse skin after UV-B irradiation, 16; 263-270 (2000)
23
M Yoshizumi, T Nakamura, M Kato, T Ishioka, K Kozawa, K Wakamatsu and H Kimura, Cell Biol. Int, Release of cytokines/ chemokines and cell death in UV-B-irradiated human keratinocytes, HaCaT, 32; 1405-1411 (2008)
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 61
  • No :3
  • Pages :184-190
  • Received Date : 2016-06-13
  • Revised Date : 2016-08-06
  • Accepted Date : 2016-08-12
  • DOI :https://doi.org/10.7740/kjcs.2016.61.3.184
Journal Informaiton The Korean Journal of Crop Science The Korean Journal of Crop Science
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close