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2018 Vol.63, Issue 2 Preview Page

Original Research Article

Identification and Quantification of Phytosterols in Maize Kernel and Cob

옥수수 종실 및 속대의 Phytosterol 동정과 함량 변이

Sun-Lim Kim1*
,
Mi-Jung Kim2
,
Gun-Ho Jung1
,
Yu-Young Lee1
,
Beom-Young Son1
,
Jung-Tae Kim1
,
Jin-Seok Lee1
,
Hwan-Hee Bae1
,
Young-Sam Go1
,
Sang-Gon Kim1
,
Seong-Bum Baek1
김 선림1*
,
김 미정2
,
정 건호1
,
이 유영1
,
손 범영1
,
김 정태1
,
이 진석1
,
배 환희1
,
고 영삼1
,
김 상곤1
,
백 성범1
1Department of Central Area Crop Science, NICS, RDA
2Research Policy Bureau of Rural Development Administration
1국립식량과학원 중부작물부
2농촌진흥청 연구정책국
*Corresponding Author
30 June 2018. pp. 131-139
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Abstract

Unsaponifiables in the kernel and the cob of 7 maize varieties were analyzed by using thin-layer chromatography (TLC) and gas chromatography (GC) for the identification of phytosterols and their concentrations. The unsaponifiables of the kernel were clearly separated into band I (campesterol, stigmasterol, and β-sitosterol), band Ⅱ (Δ5-avenasterol), band Ⅲ (Δ7- stigmastenol), and band Ⅳ (Δ7-avenasterol). In the cob, on the other hand, three or more bands were separated in addition to bands. The GC analysis of unsaponifiables showed good separation of campesterol, stigmasterol and β-sitosterol, but the mixture of Δ7- avenasterol (retention time[RT] 22.846), Δ7-stigmastenol (RT 22.852), and Δ5-avenasterol (RT 22.862) showed poor separation. Phytosterol content of the maize kernel was 635.9 mg/100 g, and that of the cob was 273.0 mg/100 g, respectively. The phytosterol content of the kernel was 2.4-fold higher than that of the cob. The phytosterol content of the kernel was higher in the order β- sitosterol 80.05% > campesterol 10.5% > stigmasterol 9.46%, but that of the cob was higher in the order β-sitosterol 59.43% > stigmasterol 31.72% > campesterol 10.98%. Based on these results, it appears that the phytosterols of the maize kernel are synthesized in the maize cob and are transferred to the kernel, because the precursors (Δ7-avenasterol, Δ7-stigmastenol, and Δ5- avenasterol) of major phytosterols were detected in maize cobs.

Keywords
cob
identification
kernel
maize
phytosterol
quantification
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 63
  • No :2
  • Pages :131-139
  • Received Date : 2018-05-26
  • Revised Date : 2018-06-07
  • Accepted Date : 2018-06-14
  • DOI :https://doi.org/10.7740/kjcs.2018.63.2.131
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