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2019 Vol.64, Issue 4 Preview Page

Original Research Article

Physiological and Proteome Responses of Korean F1 maize (Zea mays L.) Hybrids to Water-deficit Stress during Tassel Initiation

옥수수 영양생장기 한발 스트레스에 의한 광합성의 생리적 반응 및 프로테옴 변화 분석

Hwan Hee Bae1
,
Young-Sang Kwon2
,
Beom-Young Son1
,
Jung-Tae Kim1
,
Young Sam Go1
,
Sun-Lim Kim3
,
Seong-Bum Baek3
,
Seonghyu Shin1*
,
Sang Gon Kim4*
배 환희1
,
권 영상2
,
손 범영1
,
김 정태1
,
고 영삼1
,
김 선림3
,
백 성범3
,
신 성휴1*
,
김 상곤4*
1Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration
2Post Doctor, Environment Toxicology Research Center, Korea Institute of Toxicology
3Senior Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration
4Principal Researcher, Gyeongnam Oriental Anti-Aging Institute
1농촌진흥청 국립식량과학원 중부작물부 연구사
2안전성평가연구소 환경독성연구센터 박사후연구원
3농촌진흥청 국립식량과학원 중부작물부 연구관
4(재)경남한방항노화연구원 책임연구원
* Corresponding Author
31 December 2019. pp. 422-431
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Abstract

Severe droughts in spring have occurred frequently in Korea in recent years, exerting a critical impact on corn yield. Therefore, it is necessary to find physiological and/or molecular indicators of the response to drought stress in maize plants. In this study, we investigated the effects of water-deficit stress on two Korean elite F1 maize hybrids, Ilmichal and Gwangpyeongok, by withholding water for 10 days at tassel initiation. The water deficit drastically reduced the relative leaf water content, leaf number, leaf area, and stem length, leading to dry matter reduction. Moreover, it reduced the SPAD values and stomatal conductance of leaves in drought-stressed plants of both hybrids. Importantly, the number of leaves and SPAD value were non-destructive and easy to investigate in response to water-deficit stress, suggesting that they may be useful indicators for screening drought-tolerant genetic resources. We detected more than 100 spots that were differentially accumulated under drought stress. Of these spots, a total of 21 protein spots (≥1.5-fold) from drought-exposed maize leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Functional annotation using Gene Ontology analysis revealed that most of the identified proteins were involved in carbohydrate metabolism, stress response fatty acid catabolism, photosynthesis, energy metabolism, and transport. The protein expression levels were increased in both Ilmichal and Gwangpyeongok, except for triosephosphate isomerase, fructose-bisphosphate aldolase, and an uncharacterized protein. The lactoylglutathione lyase delta (3,5)-delta (2,4)- dienoyl-CoA isomerase was overexpressed in Gwangpyeongok only. The results obtained from this study suggest that the drought-specific genes may be useful as molecular markers for screening drought-tolerant maize genotypes.

Keywords
drought
Gwangpyeongok
Ilmichal
maize
protein expression
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 64
  • No :4
  • Pages :422-431
  • Received Date : 2019-09-05
  • Revised Date : 2019-10-07
  • Accepted Date : 2019-10-24
  • DOI :https://doi.org/10.7740/kjcs.2019.64.4.422
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