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2020 Vol.65, Issue 4 Preview Page
Development and Characterization of EMS-induced Mutants with Enhanced Salt Tolerance in Silage Maize

EMS 유도 내염성 증진 사료용 옥수수 돌연변이체 선발 및 특성 분석

Chuloh Cho1
,
Kyung Hwa Kim2
,
Mi-Suk Seo3
,
Man-Soo Choi3
,
Jaebuhm Chun3
,
Mina Jin4
,
Dool-Yi Kim1*
조 철오1
,
김 경화2
,
서 미숙3
,
최 만수3
,
전 재범3
,
진 민아4
,
김 둘이1*
1PostDoctor, Crop Foundation Research Division, National Institute of Crop Science, RDA
2Staff, Crop Foundation Research Division, National Institute of Crop Science, RDA
3Researcher, Crop Foundation Research Division, National Institute of Crop Science, RDA
4Senior Researcher, Crop Foundation Research Division, National Institute of Crop Science, RDA
1농촌진흥청 국립식량과학원 작물기초기반과 전문연구원
2농촌진흥청 국립식량과학원 작물기초기반과 연구원
3농촌진흥청 국립식량과학원 작물기초기반과 농업연구사
4농촌진흥청 국립식량과학원 작물기초기반과 농업연구관
*Corresponding Author
1 December 2020. pp. 406-415
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Abstract

Maize (Zea mays L.) is one of the most valuable agricultural crops and is grown under a wide spectrum of environmental conditions. However, maize is moderately sensitive to salt stress, and soil salinity is a serious threat to its production worldwide. In this study, we used ethyl methane sulfonate (EMS) to generate salt-tolerant silage maize mutants. We screened salt-tolerant lines from 203 M3 mutant populations by evaluating the morphological phenotype after salt stress treatment and selected the 140ES91 line. The 140ES91 mutant showed improved plant growth as well as higher proline content and leaf photosynthetic capacity compared with those of wild-type plants under salt stress conditions. Using whole-genome re-sequencing analysis, 1,103 single nucleotide polymorphisms and 71 insertions or deletions were identified as common variants between KS140 and 140ES91 in comparison with the reference genome B73. Furthermore, the expression patterns of three genes, which are involved in salt stress responses, were increased in the 140ES91 mutant under salt stress. Taken together, the mutant line identified in our study could be used as an improved breeding material for transferring salt tolerance traits in maize varieties.

Keywords
140ES91
EMS
mutagenesis
salt tolerance
silage maize
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :4
  • Pages :406-415
  • Received Date : 2020-08-21
  • Revised Date : 2020-10-12
  • Accepted Date : 2020-10-27
  • DOI :https://doi.org/10.7740/kjcs.2020.65.4.406
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