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2020 Vol.65, Issue 4 Preview Page
Evaluation of Tissue Culture Efficiency in a Speed Breeding System for Stable and Sustainable Supported Wheat (Triticum aestivum) Immature Embryogenesis

안정적이며 지속적 밀(Triticum aestivum) 미성숙배 조직배양을 위한 스피드 브리딩 조건의 배양 효율 검정

Geon Hee Lee1
,
Tae Kyeum Kim1
,
Chang Hyun Choi2
,
Jae Yoon Kim3*
이 건희1
,
김 태겸1
,
최 창현2
,
김 재윤3*
1Graduate Student, Department of Plant Resources, College of Industrial Science, Kongju National University
2Agriculture Researcher, National Institute of Crop Science, Rural Development Administration
3Professor, Department of Plant Resources, College of Industrial Science, Kongju National University
1국립공주대학교 식물자원학과 석사과정
2국립식량과학원 밀연구팀 농업연구사
3국립공주대학교 식물자원학과 교수
*Corresponding Author
1 December 2020. pp. 365-376
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Abstract

Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, ‘Speed Breeding’, a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety ‘Bobwhite’. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22°C. After vernalization of the Bobwhite seeds at 4°C for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

Keywords
immature embryogenesis
speed breeding
tissue culture
Triticum aestivum
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :4
  • Pages :365-376
  • Received Date : 2020-11-05
  • Revised Date : 2020-11-12
  • Accepted Date : 2020-11-13
  • DOI :https://doi.org/10.7740/kjcs.2020.65.4.365
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