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2018 Vol.63, Issue 3 Preview Page

Original Research Article

Quantification of Isoflavone Malonylglucosides in Soybean Seed during Germination

콩 발아 중 isoflavone malonylglucosides의 함량 변이

Ju-Won Lee1
,
Yoo-Jung Yi1
,
Ju-Hee Lee1
,
Min-Sik Jo1
,
Do-Jin Choi1
,
Mu-Hyun Ma1
,
Hong-Sik Kim2
,
Dae-Ok Kim3
,
Hong-Tae Yun4
,
Yong-Ho Kim1*
이 주원1
,
이 유정1
,
이 주희1
,
조 민식1
,
최 도진1
,
마 무현1
,
김 홍식2
,
김 대옥3
,
윤 홍태4
,
김 용호1*
1Dept. of Biotechnology, SoonChunHyang Univ.
2Dept. of Southern Area Crop Science, NICS, RDA
3Dept. of Food Science and Biotechnolooy, KyungHee Univ.
4Dept. of Central Area Crop Science, NICS, RDA
1순천향대학교 의료생명공학과
2국립식량과학원 남부작물부
3경희대학교 식품생명공학과
4국립식량과학원 중부작물부
*Corresponding Author
30 September 2018. pp. 239-247
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Abstract

Soybean produces three major types of isoflavones, daidzein, genistein, and glycitein aglycones and their glucosides and malonylglucosides. It has been known that malonylated glucosides are rapidly converted to their corresponding aglycones due to the unstable thermolabile glucoside malonates; therefore, the analytical study of malonylated glucosides has been insufficient. In this study, we analyzed the malonylglucoside content in soybean seeds. Isoflavone analysis of three soybean cultivars revealed that 81.5~90.0% of the total isoflavones were malonylglucosides, whereas aglycones were rarely detected. Moreover, the total isoflavone content increased during a 5-day germination period where growth regulators and coumaric acid treatments tended to yield higher isoflavone content than the normal germination treatment, however the differences were not significant; notably, the isoflavone accumulation trend continued with additional germination days. The content of malonylglucoside was higher than that of other isoflavones, which was 83.7~86.6% of the total isoflavone content in seeds with a 3-day germination period. Furthermore, isoflavones were significantly accumulated in the hypocotyl of seedlings with a 5-day germination period. The content of isoflavone in the hypocotyl of the Pungsannamul-kong was 10,240 ug/g when treated with coumaric acid, which was considerably higher than that of other cultivars and treatments. Additionally, soybean seeds heated at 60℃ for 1 hour produced higher isoflavone content than non-heated soybean seeds. Our results show that it is possible to increase the isoflavone content in soybean seeds through various treatments.

Keywords
germination
isoflavone
malonylglucoside
soybean
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 63
  • No :3
  • Pages :239-247
  • Received Date : 2018-04-30
  • Revised Date : 2018-06-18
  • Accepted Date : 2018-06-24
  • DOI :https://doi.org/10.7740/kjcs.2018.63.3.239
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