Differential Expression of Isoflavone Biosynthetic Genes in Soybean During Germination

Jin-Su Lim; Seo-Young Kim; Yong-Ho Kim

doi:10.7740/kjcs.2021.66.4.365

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2021 Vol.66, Issue 4 Preview Page Next Page

Original Research Article

Differential Expression of Isoflavone Biosynthetic Genes in Soybean During Germination 콩 발아기간 중 isoflavone 생합성 유전자 발현 변이

1 December 2021. pp. 365-374

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Abstract

Soybean isoflavones are essential secondary metabolites synthesized through the phenylpropanoid pathway, and they play vital roles in human health. Isoflavone content is a complex quantitative trait controlled by multiple genes, and the genetic mechanisms underlying isoflavone biosynthesis remain largely unknown. Therefore, the present study analyzed the content of isoflavone and expression of six key genes involved in its biosynthesis (i.e., CHS6, HID, IF7GT, IF7MaT, GmIMaT1, and GmIMaT3) during soybean seed germination. Isoflavone content was quantified using high-performance liquid chromatography, and isoflavone biosynthetic gene expression was analyzed using quantitative real-time PCR. Two cultivars, namely ‘Daepung2ho’ and ‘Pungsannamulkong’, which are high- and low-isoflavone cultivars, respectively, were used. Isoflavone accumulation gradually increased with the progression of the germination period. As such, malonyl glucosides accounted for over 80% of the total content, whereas acetyl glucosides were present at trace amounts. Transcriptional analysis of isoflavone biosynthetic genes demonstrated expression patterns parallel to isoflavone content; however, there was no clear correlation between isoflavone content and gene expression. Moreover, most isoflavone biosynthetic genes showed different expression patterns depending on the individual gene or genotypes. Among the tested genes, HID showed consistently higher expression, except at 3 days after germination, and its expression was upregulated in ‘Daepung2ho’ but downregulated in ‘Pungsannamulkong’. In addition, all tested genes exhibited different expression patterns between cotyledons and hypocotyls and responded differently to the germination period. These findings suggest that the expression levels of isoflavone biosynthetic genes are not consistent with the germination period and appear to be genotype-dependent.

Keywords

biosynthetic gene

germination

isoflavone

qRT-PCR

soybean

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Information

Publisher :The Korean Society of Crop Science
Publisher(Ko) :한국작물학회
Journal Title :The Korean Journal of Crop Science
Journal Title(Ko) :한국작물학회지
Volume : 66
No :4
Pages :365-374
Received Date : 2021-10-01
Revised Date : 2021-10-06
Accepted Date : 2021-10-07
DOI :https://doi.org/10.7740/kjcs.2021.66.4.365

The Korean Journal of Crop Science ISSN:0252-9777(Print) 2287-8432(Online) 한국작물학회지

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