All Issue

2020 Vol.65, Issue 2 Preview Page

Original Research Article

Analysis of Growth Response and Gene Expression by Waterlogging Stress on B73 Maize

침수 처리에 따른 B73 옥수수의 생육 반응 및 유전자 발현 분석

Young Sam Go1*
,
Jung-Tae Kim2
,
Hwan Hee Bae1
,
Beom-Young Son1
,
Gibum Yi3
,
Jun Young Ha4
,
Sun-Lim Kim5
,
Seong-Bum Baek5
고 영삼1*
,
김 정태2
,
배 환희1
,
손 범영1
,
이 기범3
,
하 준영4
,
김 선림5
,
백 성범5
1Agriculture Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
2Agriculture Researcher, Planning and Coordination Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea
3Post-doctor, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
4Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
5Agriculture Senior Researcher, Department of Central Area Crop Science, National Institute of Crop Science, Rural Development Administration, Suwon 16429, Korea
1농촌진흥청 국립식량과학원 중부작물부 농업연구사
2농촌진흥청 국립식량과학원 기획조정과 농업연구사
3농촌진흥청 국립식량과학원 중부작물부 전문연구원
4농촌진흥청 국립식량과학원 중부작물부 연구원
5농촌진흥청 국립식량과학원 중부작물부 농업연구관
*Corresponding Author
1 June 2020. pp. 104-112
PDF XML Citation
Abstract

Maize is thought to be an alternative crop to rice in paddy fields for efficient field management and maintenance of rice production at appropriate levels in Korea. Thus efforts to breed waterlogging-tolerant maize cultivars have been ongoing. However, molecular studies related to waterlogging tolerance are limited for developing molecular markers to select waterlogging tolerant maize varieties. In this study, we examined molecular biological changes of B73 in the V3 stage after immersion treatment for 7 days. Overall growth of maize was lower in treated samples compared to non-immersed control samples. The length of leaf and root decreased by 21.3% and 50.6%, respectively and the weight of root reduced by 21.6%. Soil plant analysis development (SPAD) value and chlorophyll content of leaf also decreased by 55.7% and 35.3%, respectively. Reactive oxygen species (ROS) content of root increased by 46.5% at 2 hours in immersion treatment. In addition, immersed roots were 2.5-fold thickened with additional aerenchyma formation in the cortex. In order to identify the causes of these changes, we performed a microarray and found increased expression of genes, such as WIP1, PMIP2, EXPA1, TPS1, and MAS1, in immersed samples. These differentially expressed genes and expression of previously reported genes, including ALDH2, Wusl1032, UP-1, UP-2, and CAT2 were further confirmed with qRT-PCR. Here, we report 7 differentially expressed genes after immersing treatment, which may be utilized as useful resources for breeding waterlogging- tolerant maize.

Keywords
B73 maize
gene expression
growth response
waterlogging
References
1
Alamgir, H. and S. N. Uddin. 2011. Mechanisms of waterlogging tolerance in wheat: morphological and metabolic adaptations under hypoxia or anoxia. Aust. J. Crop Sci. 5(9) : 940-1110.
2
Arora, K., K. K. Panda, S. Mittal, M. G. Mallikarjuna, R. R. Atmakuri, K. D. Prasanta, and T. Nepolean. 2017. RNAseq revealed the important gene pathways controlling adaptive mechanisms under waterlogged stress in maize. Sci. Rep. 7 : 10950.
10.1038/s41598-017-10561-128887464PMC5591269
3
Ashraf, M. A., M. S. Ahmad, M. Ashraf, F. Al-Qurainy, and M. Y. Ashraf. 2011. Alleviation of waterlogging stress in upland cotton (Gossypium hirsutum L.) by exogenous application of potassium in soil and as a foliar spray. Crop Pasture Sci. 62 : 25-38.
10.1071/CP09225
4
Boru, G., T. Vantoai, J. Alves, D. Hua, and M. Knee. 2003. Response of soybean to oxygen deficiency and elevated root-zone carbon dioxide concentration. Ann. Bot. 91(4) : 447-453.
10.1093/aob/mcg04012588724PMC4241062
5
Chalivendra, C. S. and M. S. Martin. 2003. Molecular and cellular adaptations of maize to flooding stress. Ann. Bot. 91(2) : 119-127.
10.1093/aob/mcf21012509333PMC4244996
6
Christianson, J. A., D. J. Llewellyn, E. S. Dennis, and I. W. Wilson. 2010. Global gene expression responses to waterlogging in roots and leaves of cotton (Gossypium hirsutum L.). Plant Cell Physiol. 51(1) : 21-37.
10.1093/pcp/pcp163
7
Gill, M. B., F. Zeng, L. Shabala, G. Zhang, M. Yu, V. Demidchik, S. Shabala, and M. Zhou. 2019. Identification of QTL related to ROS formation under hypoxia and their association with waterlogging and salt tolerance in barley. Int. J. Mol. Sci. 20(3) : 699-714.
10.3390/ijms2003069930736310PMC6387252
8
Komatsu, S., C. Han, Y. Nanjo, M. Altaf-Un-Nahar, K. Wang, D. He, and P. Yang. 2013. Label-free quantitative proteomic analysis of abscisic acid effect in early-stage soybean under flooding. J. Proteome Res. 12(11) : 4769-4784.
10.1021/pr400189823808807
9
Kong, F., A. Oyanagi, and S. Komatsu. 2010. Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS based proteomics approaches. Biochim. Biophys. Acta 1804(1) : 124-136.
10.1016/j.bbapap.2009.09.02319786127
10
Koo, S. C, H. T. Kim, B. K. Kang, Y. H. Lww, K. W. Oh, H. Y. Kim, I. Y. Back, H. T. Yun, and M. S. Choi. 2014. Screening of Flooding Tolerance in Soybean Germplasm Collection. Korean J. Breed. Sci. 46(2) : 129-135
10.9787/KJBS.2014.46.2.129
11
Liu, F., T. Vantoai, G. Bock, L.D. Linford, and J. Quackenbush. 2005. Global transcription profiling reveals novel insights into hypoxic response in Arabidopsis. Plant Physiol. 137 : 1115- 1129.
10.1104/pp.104.05547515734912PMC1065411
12
Liu, Z., K. Sunita, L. Zhang, and W. Doreen. 2012. Characterization of miRNAs in response to short-term waterlogging in three inbred lines of Zea mays. PLoS ONE 7(6) : e39786.
10.1371/journal.pone.003978622768123PMC3387268
13
Moon, J., S. Shin, H. C. Kim, K. Song, J. Y. Kim, K. Kim, and B. Lee. 2018. Assessment of the candidate genes of expression markers associated with drought stress in maize seedlings. Korean J. Breed. Sci. 50(3) : 224-235.
10.9787/KJBS.2018.50.3.224
14
Qiu, F. Z., Y. L. Zheng, Z. L. Zhang, and S. Z. Xu. 2007. Mapping of QTL associated with waterlogging tolerance during the seedling stage in maize. Ann. Bot. 99(6) : 1067-1081.
10.1093/aob/mcm05517470902PMC3244372
15
Ren, B., J. Zhang, S. Dong, P. Liu, and B. Zhao. 2016. Effects of waterlogging on leaf mesophyll cell ultrastructure and photosynthetic characteristics of summer maize. PLoS ONE 11(9) : e0161424.
10.1371/journal.pone.016142427583803PMC5008783
16
Ren, B. Z., J. W. Zhang, X. Li, X. Fan, S. T. Dong, B. Zhao, and P. Liu. 2014. Effect of waterlogging on leaf senescence characteristics of summer maize in the field. J. Appl. Ecol. 25(4) : 1022-1028.
17
Salah, A., J. Li, J. Ge, C. Cao, H. Li, Y. Wang, Z. Liu, M. Zhan, and M. Zhao. 2019. Morphological and physiological responses of maize seedlings under drought and waterlogging. J. Agr. Sci. Tech. 21(5) : 1199-1214.
18
Sallam, A. and H. D. Scott. 1987. Effects of prolonged flooding on soybeans during early vegetative growth. Soil Sci. 144(1) : 61-66.
10.1097/00010694-198707000-00010
19
Tang, B., S. Xu, X. Zou, Y. Zheng, and F. Qiu. 2010. Changes of antioxidative enzymes and lipid peroxidation in leaves and roots of waterlogging-tolerant and waterlogging-sensitive maize genotypes at seedling stage. Agric. Sci. China. 9 : 651-661.
10.1016/S1671-2927(09)60140-1
20
Yamauchi, T., I. Rajhi, and M. Nakazono. 2011. Lysigenous aerenchyma formation in maize root is confined to cortical cells by regulation of genes related to generation and scavenging of reactive oxygen species. Plant Signal. Behav. 6(5) : 759-761.
10.4161/psb.6.5.1541721502817PMC3172858
21
Yordanova, R. Y, K. G Zheng, Z. G. Stoinova, and L. P. Popova. 2004. Changes in the leaf polypeptide patterns of barley plants exposed to soil flooding. Biologia Plantarum 48(2) : 301-304.
10.1023/B:BIOP.0000033461.68221.5f
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :2
  • Pages :104-112
  • Received Date : 2020-04-12
  • Revised Date : 2020-05-07
  • Accepted Date : 2020-05-11
  • DOI :https://doi.org/10.7740/kjcs.2020.65.2.104
Journal Informaiton The Korean Journal of Crop Science The Korean Journal of Crop Science
Online Submission
  • NRF
  • KOFST
  • KISTI Current Status
  • KISTI Cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close