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2020 Vol.65, Issue 4 Preview Page
Analysis of Varietal Differences in Pre-harvest Sprouting of Rice using RNA-Sequencing

RNA-Sequencing을 이용한 벼 품종간 수발아 차이 분석

Myoung-Goo Choi1
,
Hyen-Seok Lee2
,
Woon-Ha Hwang2
,
Seo-Yeong Yang2
,
Yun-Ho Lee2
,
Chung-gun Lee3
,
Song Joong Yun4
,
Jae-Hyeok Jeong2*
최 명구1
,
이 현석2
,
황 운하2
,
양 서영2
,
이 윤호2
,
이 충근3
,
윤 성중4
,
정 재혁2*
1Post-doctoral Researcher, National Institute of Crop Science, Rural Development Administration
2(Research Scientist, National Institute of Crop Science, Rural Development Administration
3Senior Scientist, National Institute of Crop Science, Rural Development Administration
4(Professor, Department of Crop Science & Biotechnology, Chonbuk National University
1농촌진흥청 국립식량과학원 전문연구원
2농촌진흥청 국립식량과학원 농업연구사
3농촌진흥청 국립식량과학원 농업연구관
4전북대학교 농업생명과학대학 작물생명과학과 교수
*Corresponding Author
1 December 2020. pp. 274-283
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Abstract

Seed dormancy is an adaptive trait in which seeds do not germinate under unfavorable environmental conditions. Low dormancy seeds are easily germinated under optimal environmental conditions, and these characteristics greatly reduce the yield and quality of crops. In the present study, we compared the pre-harvest sprouting (PHS) rate of two cultivars, Joun and Jopyeong, using the Winkler scale after heading day and temperature of the test. The PHS rate increased as the Winkler scale after heading day increased from 700°C to 1100°C and the temperature of the test increased. In all conditions, the PHS rate of Jopyeong was higher than that of Joun. RNA-sequencing was used to analyze the cause of the high PHS rate. We analyzed the biological metabolic processes related to the abscisic acid (ABA) metabolite pathway using the KEGG mapper with selected differentially expressed genes in PHS seeds. We found that the expression of ABA biosynthesis genes (OsNCEDs) was down-regulated and that ABA catabolic genes (OsCYP707As) was up-regulated in PHS seeds. However, the quantitative real-time PCR results showed that Joun had a higher expression of OsNCEDs than that of Jopyeong, but OsCYP707As did not yield a significant result. Joun displayed higher ABA content than that of Jopyeong not only during ripeness time but also during PHS treatment. Taken together, we provided evidence that the ABA content remaining in the seed is important to the PHS rate, which is determined by the expression level of the ABA biosynthesis gene OsNCEDs.

Keywords
ABA
pre-harvest sprouting
rice
RNA-sequencing
References
1
Anders, S. and W. Huber. 2010. Differential expression analysis for sequence count data. Nature Precedings. 10.1038/npre.2010.4282.1
2
Ashburner, M., C. A. Ball, J. A. Blake, D. Botstein, H. Butler, J. M. Cherry, et al. 2000. Gene Ontology: tool for the unification of biology. Nature Genetics 25(1) : 25-29. 10.1038/7555610802651PMC3037419
3
Baek, J. and N. Chung. 2014. Pre-harvest sprouting variation of rice seeds located on each panicle position according to grain filling days. Korean Journal of Crop Science/Hanguk Jakmul Hakhoe Chi. 59(1) : 22-26. 10.7740/kjcs.2014.59.1.022
4
Chono, M., I. Honda, S. Shinoda, T. Kushiro, Y. Kamiya, E. Nambara, N. Kawakami, S. Kaneko, and Y. Watanabe. 2006. Field studies on the regulation of abscisic acid content and germinability during grain development of barley: molecular and chemical analysis of pre-harvest sprouting. J. Exp. Bot. 57(10) : 2421-2434. 10.1093/jxb/erj21516798848
5
Fang, J., C. Chai, Q. Qian, C. Li, J. Tang, L. Sun, Z. Huang, X. Guo, C. Sun, and M. Liu. 2008. Mutations of genes in synthesis of the carotenoid precursors of ABA lead to pre‐harvest sprouting and photo‐oxidation in rice. The Plant Journal 54(2) : 177-189. 10.1111/j.1365-313X.2008.03411.x18208525PMC2327239
6
Hu, Q., C. Lin, Y. Guan, M. S. Sheteiwy, W. Hu, and J. Hu. 2017. Inhibitory effect of eugenol on seed germination and pre-harvest sprouting of hybrid rice (Oryza sativa L.). Sci Rep-Uk. 7(1) : 5295. 10.1038/s41598-017-04104-x28706198PMC5509651
7
Ju, Y. C., S. W. Han, J. S. Park, and K. Y. Park. 2000. Effective screening method for viviparous germination of rice. Korean Journal of Crop Science 45(2) : 103-107.
8
Kang, S., J. Shon, H. Kim, S. Kim, J. Choi, J. Park, J. Sim, and W. Yang. 2018. Analysis of genetic variation in pre-harvest sprouting at different cumulative temperatures after heading of rice. Korean Journal of Crop Science 63(1) : 8-17.
9
Kim, S.-J., J.-G. Won, D.-J. Ahn, S.-D. Park, and C.-D. Choi. 2008. Influence of viviparous germination on quality and yield in rice. Korean Journal of Crop Science 53(spc) : 15-18.
10
Ko, J.-C., B.-k. Kim, K.-S. Lee, W.-Y. Choi, H.-R. Choi, E. Cho, and S.-J. Yu. 2005. Varietal difference in enzyme activities during preharvest germination of rice. Korean Journal of Crop Science 50(6) : 378-383.
11
Kucera, B., M. A. Cohn, and G. Leubner-Metzger. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Science Research 15(4) : 281-307. 10.1079/SSR2005218
12
Lee, M., S. Park, and R. Park. 1985. Effect of pre-harvest sprouting on yield and grain quanlity in rice. Annual Res Rep NICS, RDA, pp. 477-480.
13
Liu, Y., J. Fang, F. Xu, J. Chu, C. Yan, M. R. Schlappi, Y. Wang, and C. Chu. 2014. Expression patterns of ABA and GA metabolism genes and hormone levels during rice seed development and imbibition: A comparison of dormant and non-dormant rice cultivars. J. Genet. Genomics. 41(6) : 327-338. 10.1016/j.jgg.2014.04.00424976122
14
Oh, S., C. Kim, C. Kim, S. Kim, and J. Lee. 1987. Influence of viviparous germination on quality and yeild potential of rice. Res Rep RDA (Crops). 29(1) : 68-73.
15
Park, K. B. and R. K. Park. 1984. Studies on the viviparous germination of indica × japonica type varieties in paddy rice. Korean Journal of Crop Science/Hanguk Jakmul Hakhoe Chi. 29(1) : 15-18.
16
Park, J.-S. and H.-D. Kim. 2009. Viviparous germination characteristics of rice varieties adaptable to central region of Korea. Korean Journal of Crop Science 54(3) : 241-248.
17
Shon, J., J. Kim, H. Jung, B. Kim, K. Choi, and W. Yang. 2014. Effect of pre-harvest sprouting on seed viability, germination and seedling emergence rate of rice. Korean Journal of Crop Science/Hanguk Jakmul Hakhoe Chi. 59(4) : 427-434. 10.7740/kjcs.2014.59.4.427
18
Suh, K. and Y. Kim. 1994. Varietal difference in viviparous germination at different days after heading and temperature conditions in rice. Korean Journal of Crop Science 32(2) : 187-192.
19
Shu, K., H. Zhang, S. Wang, M. Chen, Y. Wu, S. Tang, C. Liu, Y. Feng, X. Cao, and Q. Xie. 2013. ABI4 regulates primary seed dormancy by regulating the biogenesis of abscisic acid and gibberellins in Arabidopsis. PLoS Genetics 9(6) : e1003577. 10.1371/journal.pgen.100357723818868PMC3688486
20
Tuan, P. A., R. Kumar, P. K. Rehal, P. K. Toora, and B. T. Ayele. 2018. Molecular mechanisms underlying abscisic acid/gibberellin balance in the control of seed dormancy and germination in cereals. Front Plant Sci. 9 : 668. 10.3389/fpls.2018.0066829875780PMC5974119
21
Walker-Simmons, M. 1987. ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars. Plant Physiology. 84(1) : 61-66. 10.1104/pp.84.1.6116665406PMC1056528
22
Zhu, G., N. Ye, and Zhang, J. 2009. Glucose-Induced Delay of Seed Germination in Rice is Mediated by the Suppression of ABA Catabolism Rather Than an Enhancement of ABA Biosynthesis. Plant and Cell Physiology 50(3) : 644-651. 10.1093/pcp/pcp02219208695
Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :4
  • Pages :274-283
  • Received Date : 2020-09-20
  • Revised Date : 2020-11-02
  • Accepted Date : 2020-11-02
  • DOI :https://doi.org/10.7740/kjcs.2020.65.4.274
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