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2021 Vol.66, Issue 4 Preview Page

Original Research Article

Development of EST-SSRs and Assessment of Genetic Diversity in Germplasm of the Finger Millet, Eleusine coracana (L.) Gaertn.
Xiaohan Wang1
,
Myung Chul Lee1
,
Yu-Mi Choi1
,
Seong-Hoon Kim1
,
Seahee Han2
,
Kebede Taye Desta13
,
Hye-myeong Yoon1
,
Yoonjung Lee1
,
Miae Oh1
,
Jung Yoon Yi1
,
Myoung-Jae Shin1*
,
Kyung-Min Kim4*
1Junior Scientist, National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
2Associate Researcher, Honam National Institute of Biological Resources, Mokpo 58762, Korea
3Professor, Department of Applied Chemistry, Adama Science and Technology University, Adama 1888, Ethiopia
4Professor, School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu 41566, Korea
*Corresponding Author
1 December 2021. pp. 443-451
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Abstract

Finger millet (Eleusine coracana) is widely cultivated in tropical regions worldwide owing to its high nutritional value. Finger millet is more tolerant against biotic and abiotic stresses such as pests, drought, and salt than other millet crops; therefore, it was proposed as a candidate crop to adapt to climate change in Korea. In 2019, we used expressed sequence tag simple sequence repeat (EST-SSR) markers to evaluate the genetic diversity and structure of 102 finger millet accessions from two geographical regions (Africa and South Asia) to identify appropriate accessions and enhance crop diversity in Korea. In total, 40 primers produced 116 alleles, ranging in size from 135 to 457 bp, with a mean polymorphism information content (PIC) of 0.18225. Polymorphism was detected among the 40 primers, and 13 primers were found to have PIC values > 0.3. Principal coordinate and phylogenetic analyses, based on the combined data of both markers, grouped the finger millet accessions according to their respective collection areas.Therefore, the 102 accessions were classified into two groups, one from Asia and the other from Africa. We have conducted an in-depth study on the finger millet landrace pedigree. By sorting out and using the molecular characteristics of each pedigree, it will be useful for the management and accession identification of the plant resource. The novel SSR markers developed in this study will aid in future genetic analyses of E. coracana.

Keywords
phylogenetic analysis
population structure
selfing plants
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 66
  • No :4
  • Pages :443-451
  • Received Date : 2021-10-09
  • Revised Date : 2021-11-01
  • Accepted Date : 2021-11-17
  • DOI :https://doi.org/10.7740/kjcs.2021.66.4.443
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