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2020 Vol.65, Issue 3

Original Research Article

Improvement of Seedling Establishment in Wet Direct Seeding of Rice using the Anaerobic Germination Tolerance Gene Derived from Weedy Photoblastic Rice

잡초벼 PBR 혐기발아 내성 유전자 활용 벼 담수직파 초기 입모 개선

Jong-Min >Jeong1*
,
Youngjun Mo1
,
Man-Kee Baek1
,
Woo-Jae Kim2
,
Young-Chan Cho3
,
Su-Kyung Ha1
,
Jinhee Kim4
,
Ji-Ung Jeung3
,
Suk-Man Kim4*
정 종민1*
,
모 영준1
,
백 만기1
,
김 우재2
,
조 영찬3
,
하 수경1
,
김 진희4
,
정 지웅3
,
김 석만4*
1Junior Researcher, Crop breeding Division, National Institute of Crop Science, Rural Development Administration
2Junior Researcher, International Technology Cooperation Division, Rural Development Administration
3Senior Researcher, Crop breeding Division, National Institute of Crop Science, Rural Development Administration
4Postdoctoral Researcher, Crop breeding Division, National Institute of Crop Science, Rural Development Administration
1농촌진흥청 국립식량과학원 작물육종과 농업연구사
2농촌진흥청 국제기술협력과 농업연구사
3농촌진흥청 국립식량과학원 작물육종과 농업연구관
4농촌진흥청 국립식량과학원 작물육종과 전문연구원
* Corresponding Author
1 September 2020. pp. 161-171
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Abstract

Direct seeding is one of the rice seedling establishment methods that is increasingly being practiced by farmers to save labor and reduce costs. However, this method often causes poor germination under flooding conditions after sowing. In this study, we developed japonica elite lines with quantitative trait loci (QTL) associated with anaerobic germination (AG) tolerance to overcome poor germination and seedling establishment in wet direct seeding. The QTL introgression lines were developed from a cross between weedy photoblastic rice as the AG donor and the Nampyeong variety via phenotypic and genotypic selection. Compared to Nampyeong, the survival rates of the selected lines were improved by approximately 50% and 240% under field and greenhouse conditions, respectively. To improve selection efficiency by marker assisted selection, the QTL markers associated with AG tolerance were converted to cleaved amplified polymorphic sequence markers designed based on next-generation sequence analysis. These lines retained similar agronomic traits and yield potential to the parent, Nampyeong. Among these lines, we selected the most promising line, which exhibited high survival rate and good agricultural traits under flooding conditions and named the line as Jeonju643. This line will contribute to breeding programs aiming to develop rice cultivars adapted to wet direct seeding. This study demonstrates the successful application of marker-assisted selection to targeted introgression of anaerobic genes into a premium quality japonica rice variety.

Keywords
anaerobic germination (AG)
breeding
direct seeding
DNA markers
rice
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 65
  • No :3
  • Pages :161-171
  • Received Date : 2020-04-02
  • Revised Date : 2020-05-14
  • Accepted Date : 2020-06-18
  • DOI :https://doi.org/10.7740/kjcs.2020.65.3.161
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