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2019 Vol.64, Issue 1 Preview Page

Original Research Article

Influence of Abiotic Treatments on Isoflavone Accumulation in Soybean Seeds during Germination

물리적 요인이 콩 발아 중 isoflavone 함량에 미치는 영향

Min-Sik Jo1
,
Ju-Hee Lee1
,
Mu-Hyun Ma1
,
Seo-Young Kim1
,
Chea-Rim Byun1
,
Yoo-Jung Yi1
,
Ju-Won Lee1
,
Do-Jin Choi1
,
Hong-Sik Kim2
,
Yong-Ho Kim3*
조 민식1
,
이 주희1
,
마 무현1
,
김 서영1
,
변 채림1
,
이 유정1
,
이 주원1
,
최 도진1
,
김 홍식2
,
김 용호3*
1Student, Dept. of Biotechnology, SoonChunHyang Univ., Asan-si 31538, Korea
2Senior Researcher, Dept. of Southern Area Crop Science, NICS, RDA, Miryang 50424, Korea
3Professor, Dept. of Biotechnology, SoonChunHyang Univ., Asan-si 31538, Korea
1순천향대학교 의료생명공학과 학생
2국립식량과학원 남부작물부 농업연구관
3순천향대학교 의료생명공학과 교수
*Corresponding Author
31 March 2019. pp. 18-24
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Abstract

Isoflavones, a group of secondary metabolites present in plants and especially abundant in soybeans, are garnering increasing interest because of their medicinal properties. Numerous environmental factors influence isoflavone accumulation in plants. Successful attempts to increase isoflavone contents in soybean seeds have not been widely reported. In this report, we examined an effective method for producing a high amount of isoflavones during soybean seed germination, by treating the seed with 4 abiotic factors. Soybean seeds were exposed to ultrasound (50 Hz) and heat (40°C) for 30minutes, then germinated for 5 days in darkness. Another sample was subjected to water deficit during germination in darkness, and the other sample was subjected to light conditions during the germination period. The results showed that the ultrasound treatment increased isoflavone content during soybean germination. Three days after germination, the ultrasound-treated seeds presented the highest isoflavone content (6,042.1 ug/g dry weight) compared to the other treatments, which was also 1.3-fold increases over the content of the raw seed’s before germination. With respect to each type of isoflavone group, aglycone (partially genistein) content was markedly higher, whereas glucoside was lower than that of the untreated samples. In other experiment, total isoflavone content after heat, water deficit, and light treatments was 5,600.0, 4,740.1, and 5,631.4 ug/g, respectively. These present work suggests that ultrasound treatment is a novel approach for improving the production of isoflavones in soybean seeds during germination.

Keywords
abiotic
germination
isoflavone
soybean
ultrasound
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Information
  • Publisher :The Korean Society of Crop Science
  • Publisher(Ko) :한국작물학회
  • Journal Title :The Korean Journal of Crop Science
  • Journal Title(Ko) :한국작물학회지
  • Volume : 64
  • No :1
  • Pages :18-24
  • Received Date : 2019-03-11
  • Revised Date : 2019-03-21
  • Accepted Date : 2019-03-21
  • DOI :https://doi.org/10.7740/kjcs.2019.64.1.018
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